The impact of delayed blood centrifuging, choice of collection tube, and type of assay on 25-hydroxyvitamin D concentrations

C L Yu; R T Falk; Michael G Kimlin; P Rajaraman; A J Sigurdson; R L Horst; L M Cosentino; M S Linet; D M Freedman

doi:10.1007/s10552-009-9485-x

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The impact of delayed blood centrifuging, choice of collection tube, and type of assay on 25-hydroxyvitamin D concentrations

Journal article

Open access

Peer reviewed

The impact of delayed blood centrifuging, choice of collection tube, and type of assay on 25-hydroxyvitamin D concentrations

C L Yu, R T Falk, Michael G Kimlin, P Rajaraman, A J Sigurdson, R L Horst, L M Cosentino, M S Linet and D M Freedman

Cancer Causes and Control, Vol.21(4), pp.643-648

2010

DOI: https://doi.org/10.1007/s10552-009-9485-x

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Abstract

vitamin D

5-hydroxyvitamin D

specimen handling

time factor

epidemiologic methods

Studies have examined the associations between cancers and circulating 25-hydroxyvitamin D [25(OH)D], but little is known about the impact of different laboratory practices on 25(OH)D concentrations. We examined the potential impact of delayed blood centrifuging, choice of collection tube, and type of assay on 25(OH)D concentrations. Blood samples from 20 healthy volunteers underwent alternative laboratory procedures: four centrifuging times (2, 24, 72, and 96 h after blood draw); three types of collection tubes (red top serum tube, two different plasma anticoagulant tubes containing heparin or EDTA); and two types of assays (DiaSorin radioimmunoassay [RIA] and chemiluminescence immunoassay [CLIA/LIAISON® Log-transformed 25(OH)D concentrations were analyzed using the generalized estimating equations (GEE) linear regression models. We found no difference in 25(OH)D concentrations by centrifuging times or type of assay. There was some indication of a difference in 25(OH)D concentrations by tube type in CLIA/LIAISON®-assayed samples, with concentrations in heparinized plasma (geometric mean, 16.1 ng ml-1) higher than those in serum (geometric mean, 15.3 ng ml-1) (p = 0.01), but the difference was significant only after substantial centrifuging delays (96 h). Our study suggests no necessity for requiring immediate processing of blood samples after collection or for the choice of a tube type or assay. © 2009 US Government.

Details

Title: The impact of delayed blood centrifuging, choice of collection tube, and type of assay on 25-hydroxyvitamin D concentrations
Authors: C L Yu (Author) - National Institute of Health, United States
R T Falk (Author) - National Institute of Health, United States
Michael G Kimlin (Author) - Queensland University of Technology
P Rajaraman (Author) - National Institute of Health, United States
A J Sigurdson (Author) - National Institute of Health, United States
R L Horst (Author) - Heartland Assays, Inc, United States
L M Cosentino (Author) - SAIC-Frederick Inc., United States
M S Linet (Author) - National Institute of Health, United States
D M Freedman (Author) - National Institute of Health, United States
Publication details: Cancer Causes and Control, Vol.21(4), pp.643-648
Publisher: Springer Netherlands
Date published: 2010
DOI: 10.1007/s10552-009-9485-x
ISSN: 0957-5243
Copyright note: Copyright © 2010 Springer Netherlands. The author's accepted version is reproduced here in accordance with the publisher's copyright policy. The final publication is available at Springer via http://dx.doi.org/10.1007/s10552-009-9485-x
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy
Language: English
Record Identifier: 99449254502621
Output Type: Journal article

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