Journal article
The impact of Acute Lung Injury, ECMO and transfusion on oxidative stress and plasma selenium levels in an ovine model
Journal of Trace Elements in Medicine and Biology, Vol.30, pp.4-10
2015
Abstract
The purpose of this study was to determine the effects of smoke induced acute lung injury (S-ALI), extracorporeal membrane oxygenation (ECMO) and transfusion on oxidative stress and plasma selenium levels. Forty ewes were divided into (i) healthy control (n = 4), (ii) S-ALI control (n = 7), (iii) ECMO control (n = 7) (iv) S-ALI + ECMO (n = 8) and (v) S-ALI + ECMO + packed red blood cell (PRBC) transfusion (n = 14). Plasma thiobarbituric acid reactive substances (TBARS), selenium and glutathione peroxidase (GPx) activity were analysed at baseline, after smoke injury (or sham) and 0.25, 1, 2, 6, 7, 12 and 24 hours after initiation of ECMO. Peak TBARS levels were similar across all groups. Plasma selenium decreased by 54% in S-ALI sheep (1.36±0.20 to 0.63±0.27 μmol/L, p < 0.0001), and 72% in sheep with S-ALI + ECMO at 24hr (1.36±0.20 to 0.38±0.19, p < 0.0001). PRBC transfusion had no effect on TBARS, selenium levels or glutathione peroxidase activity in plasma. While ECMO independently increased TBARS in healthy sheep to levels which were similar to the S-ALI control, the addition of ECMO after S-ALI caused a negligible increase in TBARS. This suggests that the initial lung injury was the predominant feature in the TBARS response. In contrast, the addition of ECMO in S-ALI sheep exacerbated reductions in plasma selenium beyond that of S-ALI or ECMO alone. Clinical studies are needed to confirm the extent and duration of selenium loss associated with ECMO.
Details
- Title
- The impact of Acute Lung Injury, ECMO and transfusion on oxidative stress and plasma selenium levels in an ovine model
- Authors
- Charles I McDonald (Author) - University of QueenslandYoke Lin Fung (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringKiran Shekar (Author) - University of QueenslandSara D Diab (Author) - University of QueenslandKimble R Dunster (Author) - University of QueenslandMargaret R Passmore (Author) - University of QueenslandSamuel R Foley (Author) - University of QueenslandGabriela Simonova (Author) - University of QueenslandDavid Platts (Author) - University of QueenslandJohn F Fraser (Author) - University of Queensland
- Publication details
- Journal of Trace Elements in Medicine and Biology, Vol.30, pp.4-10
- Publisher
- Urban & Fischer Verlag
- Date published
- 2015
- DOI
- 10.1016/j.jtemb.2015.01.004
- ISSN
- 0946-672X
- Copyright note
- Copyright © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99450062202621
- Output Type
- Journal article
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