Journal article
The characteristics of genetically related Pseudomonas aeruginosa from diverse sources and their interaction with human cell lines
Canadian Journal of Microbiology, Vol.62(3), pp.233-240
2016
PMID: 26854365
Abstract
We investigated a collection of Pseudomonas aeruginosa strains from hospitalized patients (n=20) and various environmental sources (n=214) for their genetic relatedness, virulence properties, antibiotic resistance and interaction with intestinal (Caco-2), renal (A-498) and lung (Calu-3) cell lines. Using RAPD-PCR, high diversity was found amongst the strains irrespective of their sources, with only six common (C) types containing strains from both a clinical and environmental source. Environmental strains belonging to these C-types showed higher adhesion to A-498 cells than clinical strains (17±13 bacteria/cell versus 13±11 bacteria/cell; p<0.001), whereas clinical strains adhered significantly greater to Calu-3 and Caco-2 cells than environmental strains (p<0.001 for both). The virulence genes (VGs) and antibiotic resistance profiles of the strains were similar, however the prevalence of environmental strains carrying both exoS and exoU was significantly (p<0.0368) higher than clinical strains. Whilst all strains were resistant to ticarcillin and ticarcillin/clavulanic acid, resistance against aztreonam, gentamicin, amikacin, piperacillin and ceftazidime varied among environmental and clinical strains. These results suggest that environmental strains of P. aeruginosa carry similar virulence properties to clinical strains, including adhesion to various human cell lines with some strains showing a higher adhesion to specific cell lines which indicate they may have a better ability to cause infection in those sites under predisposing conditions of the host.
Details
- Title
- The characteristics of genetically related Pseudomonas aeruginosa from diverse sources and their interaction with human cell lines
- Authors
- Klrissa Streeter (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringChristina Neuman (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringJasmin M Thompson (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringEva Hatje (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Canadian Journal of Microbiology, Vol.62(3), pp.233-240
- Publisher
- N R C Research Press
- Date published
- 2016
- DOI
- 10.1139/cjm-2015-0536
- ISSN
- 0008-4166
- PMID
- 26854365
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99449356402621
- Output Type
- Journal article
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- Biochemistry & Molecular Biology
- Biotechnology & Applied Microbiology
- Immunology
- Microbiology
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