The Variability in Type I Collagen Helical Pitch Is Reflected in the D Periodic Fibrillar Structure

G J Cameron; D E Cairns; Tim J Wess

doi:10.1016/j.jmb.2007.05.076

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The Variability in Type I Collagen Helical Pitch Is Reflected in the D Periodic Fibrillar Structure

Journal article

Peer reviewed

The Variability in Type I Collagen Helical Pitch Is Reflected in the D Periodic Fibrillar Structure

G J Cameron, D E Cairns and Tim J Wess

Journal of Molecular Biology, Vol.372(4), pp.1097-1107

2007

DOI: https://doi.org/10.1016/j.jmb.2007.05.076

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Abstract

type I collagen

genetic algorithm

fibril

structure

X-ray

The variability in amino acid axial rise per residue of the collagen helix is a potentially important parameter that is missing in many structural models of fibrillar collagen to date. The significance of this variability has been supported by evidence from collagen axial structures determined by electron microscopy and X-ray diffraction, as well as studies of the local sequence-dependent conformation of the collagen helix. Here, sequence-dependent variation of the axial rise per residue was used to improve the fit between simulated diffraction patterns derived from model structures of the axially projected microfibrillar structure and the observed X-ray diffraction pattern from hydrated rat tail tendon. Structural models were adjusted using a genetic algorithm that allowed a wide range of structures to be tested efficiently. The results show that variation of the axial rise per residue could reduce the difference metric between model and observed data by up to 50%, indicating that such a variable is a necessary part of fibril model structure building. The variation in amino acid translation was also found to be influenced by the number of proline and hydroxyproline residues in the triple helix structure. © 2007 Elsevier Ltd. All rights reserved.

Details

Title: The Variability in Type I Collagen Helical Pitch Is Reflected in the D Periodic Fibrillar Structure
Authors: G J Cameron (Author) - University of Stirling, United Kingdom
D E Cairns (Author) - University of Stirling, United Kingdom
Tim J Wess (Author) - University of Stirling, United Kingdom
Publication details: Journal of Molecular Biology, Vol.372(4), pp.1097-1107
Publisher: Academic Press
Date published: 2007
DOI: 10.1016/j.jmb.2007.05.076
ISSN: 0022-2836; 0022-2836
Organisation Unit: Office of the Deputy Vice-Chancellor (Academic); University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99451462102621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemistry & Molecular Biology