The Value and Clinical Significance of ZNF582 Gene Methylation in the Diagnosis of Cervical Cancer

Chunhe Zhang; Shaowei Fu; Luyue Wang; Fang Wang; Dan Wu; Xiangyi Zhe; Huizhen Xin; Hongtao Li; Dongmei Li; Fuyuan Jin; Renfu Shao; Zemin Pan

doi:10.2147/OTT.S277445

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The Value and Clinical Significance of ZNF582 Gene Methylation in the Diagnosis of Cervical Cancer

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The Value and Clinical Significance of ZNF582 Gene Methylation in the Diagnosis of Cervical Cancer

Chunhe Zhang, Shaowei Fu, Luyue Wang, Fang Wang, Dan Wu, Xiangyi Zhe, Huizhen Xin, Hongtao Li, Dongmei Li, Fuyuan Jin, …

OncoTargets and Therapy, Vol.14, pp.403-411

2021

DOI: https://doi.org/10.2147/OTT.S277445

PMCID: PMC7814240

PMID: 33488095

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Abstract

HPV16/18

promoter methylation

cervical cancer

ZNF582 gene

gene expression

Introduction: The aim of this study was to determine whether ZNF582 gene methylation and tissue protein expression can be used as a tool with high sensitivity and specificity for cervical cancer screening. We analyzed the correlation between promoter methylation of ZNF582 gene and cervical cancer and high risk HPV16/18 infection. Methods: Tissue samples of normal cervical or chronic cervicitis (n=51), CIN (cervical intraepithelial neoplasia) (n=35), and cervical carcinoma (n=68) were tested for HPV16/18 infection by polymerase chain reaction (PCR). We also detected the methylation status of the ZNF582 gene promoter in the same tissues by methylation-specific PCR (MSP), then analyzed the correlation between ZNF582 promoter methylation and HPV16/18 infection. Immunohistochemistry was used to analyze ZNF582 gene expression in 152 cervical tissues. We detected ZNF582 mRNA expression in cervical tissues (including cancer and non-cancer) by real-time fluorescence quantitative PCR (qPCR). Results: Among 93 high-grade cervical lesions (CINII and above) and cervical cancer samples, 57 cases were positive for HPV16/18 infection and 36 cases were negative. ZNF582 gene methylation occurred in 9 out of 51 cases in normal cervical tissues (17.6%), 16 of 35 cases in CIN tissues (45.7%), and 50 of 68 cases in cervical cancer (73.5%). The differences in methylation rate of the three groups were statistically significant (P< 0.05). The ZNF582 methylation rate in the positive HPV16/18 infection group was 73.7%, while the negative group was 63.9%. Compared with normal tissues, ZNF582 protein was highly expressed in cervical cancer tissues, but mRNA expression was low. Conclusion: While ZNF582 protein is highly expressed in cervical cancer tissues, it was not sufficient for use as a standard for cervical cancer staging. On the other hand, ZNF582 promoter methylation had high specificity and sensitivity in detecting CINII and highly diseased cervical lesions and could be used as a diagnostic marker for cervical cancer of women.

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Title: The Value and Clinical Significance of ZNF582 Gene Methylation in the Diagnosis of Cervical Cancer
Authors: Chunhe Zhang (Author) - Shihezi University
Shaowei Fu (Author) - Shihezi University
Luyue Wang (Author) - Shihezi University
Fang Wang (Author) - Shihezi University
Dan Wu (Author) - Shihezi University
Xiangyi Zhe (Author) - Shihezi University
Huizhen Xin (Author) - Shihezi University
Hongtao Li (Author) - Shihezi University
Dongmei Li (Author) - Shihezi University
Fuyuan Jin (Author) - Shihezi University
Renfu Shao (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - Legacy
Zemin Pan (Author) - Shihezi University
Publication details: OncoTargets and Therapy, Vol.14, pp.403-411
Publisher: Dove Medical Press Ltd.
Date published: 2021
DOI: 10.2147/OTT.S277445
ISSN: 1178-6930
PMID: 33488095; PMC7814240
Copyright note: © 2021 Zhang et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php)
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99506007402621
Output Type: Journal article

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