The Src kinase Lyn is a negative regulator of mast cell proliferation

Valerie Hernandez-Hansen; Graham A Mackay; Clifford A Lowell; Bridget S Wilson; Janet M Oliver

doi:10.1189/jlb.0503224

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The Src kinase Lyn is a negative regulator of mast cell proliferation

Journal article

Peer reviewed

The Src kinase Lyn is a negative regulator of mast cell proliferation

Valerie Hernandez-Hansen, Graham A Mackay, Clifford A Lowell, Bridget S Wilson and Janet M Oliver

Journal of Leukocyte Biology, Vol.75(1), pp.143-151

2004

DOI: https://doi.org/10.1189/jlb.0503224

PMID: 14525964

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Abstract

Signal Transduction

mast cells/basophils

apoptosis

cellular proliferation

cellular activation

Previous investigators have reported that deletion of the protein tyrosine kinase Lyn alters mast cell (MC) signaling responses but does not affect or reduces the cytokine-mediated proliferation of mouse bone marrow-derived MC (BMMC) precursors and of mature MC. We observed that Lyn-deficient mice have more peritoneal MC than wild-type (WT) mice. Studies to explore this unexpected result showed that Lyn(-/-) BM cells expand faster than WT cells in response to interleukin (IL)-3 and stem-cell factor over the 4-5 weeks required to produce a >95% pure population of granular, receptor with high affinity for immunoglobulin E-positive BMMC. Furthermore, differentiated Lyn(-/-) BMMC continue to proliferate more rapidly than WT BMMC and undergo less apoptosis in response to cytokine withdrawal. Additionally, Lyn(-/-) BMMC support greater IL-3-mediated phosphorylation of the prosurvival kinase, Akt, and the proliferative kinase, extracellular-regulated kinase 1/2. These results identify Lyn as a negative regulator of murine MC survival and proliferation.

Details

Title: The Src kinase Lyn is a negative regulator of mast cell proliferation
Authors: Valerie Hernandez-Hansen (Author) - University of New Mexico
Graham A Mackay (Author) - University of New Mexico
Clifford A Lowell (Author) - University of California, San Francisco
Bridget S Wilson (Author) - University of New Mexico
Janet M Oliver (Author) - University of New Mexico
Publication details: Journal of Leukocyte Biology, Vol.75(1), pp.143-151
Publisher: John Wiley & Sons Ltd.
DOI: 10.1189/jlb.0503224
ISSN: 1938-3673
PMID: 14525964
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99522303502621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Cell Biology; Hematology; Immunology

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