Journal article
The Preventative Effects of Procyanidin on Binge Ethanol-Induced Lipid Accumulation and ROS Overproduction via the Promotion of Hepatic Autophagy
Molecular Nutrition & Food Research, Vol.63(18), pp.1-12
2019
PMID: 31336037
Abstract
Scope
Autophagy plays an important role in alleviating alcoholic liver disease (ALD). In this study, it is discovered that a dimer procyanidin (DPC) significantly prevented ALD by promoting hepatic autophagy.
Methods and results
Both cell and animal disease models stimulated by excessive ethanol are employed to evaluate the protective actions of DPC. Specifically, in vitro, DPC significantly decreased intracellular lipid deposition, diminished reactive oxygen species (ROS) formation, and elevated the level of mitochondrial membrane potential. These beneficial effects can be remarkably blocked by 3-methyladenine, a potent autophagy inhibitor, suggesting the autophagy-dependent protective role of DPC. In vivo, DPC pretreatment can also significantly reduce lipid accumulation, ROS overproduction, and elevated GSH content in the liver. Similarly, these protective effects of DPC can be partially reversed by chloroquine, a lysosomal inhibitor used to block the late-stage autophagy flux. Moreover, the determinations of LC3 and p62 protein expressions, autophagic flux assessments, and transmission electron microscopy observation further demonstrate the pro-autophagic effect of DPC.
Conclusions
DPC may activate hepatic autophagy to eliminate lipid droplets and damaged mitochondria, thereby reducing hepatic lipid disposition and ROS overproduction. This study demonstrates that DPC is a protective reagent on ALD, providing a novel strategy of fighting ALD.
Details
- Title
- The Preventative Effects of Procyanidin on Binge Ethanol-Induced Lipid Accumulation and ROS Overproduction via the Promotion of Hepatic Autophagy
- Authors
- Peng Cao - Huazhong University of Science and TechnologyYu Zhang - Huazhong University of Science and TechnologyZi Huang - Huazhong University of Science and TechnologyMitchell A Sullivan - University of QueenslandZihao He - Huazhong University of Science and TechnologyJinglin Wang - Huazhong University of Science and TechnologyHuiping Hu - Huazhong University of Science and TechnologyKaiping Wang (Corresponding Author) - Huazhong University of Science and Technology
- Publication details
- Molecular Nutrition & Food Research, Vol.63(18), pp.1-12
- Publisher
- Wiley-VCH Verlag GmbH & Co. KGaA
- Date published
- 2019
- DOI
- 10.1002/mnfr.201801255
- ISSN
- 1613-4133
- PMID
- 31336037
- Organisation Unit
- School of Health - Biomedicine
- Language
- English
- Record Identifier
- 991035095902621
- Output Type
- Journal article
Metrics
4 Record Views
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- Food Science & Technology
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Source: InCites