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The Predatory Stink Bug Arma custos (Hemiptera Pentatomidae) Produces a Complex Proteinaceous Venom to Overcome Caterpillar Prey5.44 MB
Published VersionCC BY V4.0, Open Access
Journal article
The Predatory Stink Bug Arma custos (Hemiptera: Pentatomidae) Produces a Complex Proteinaceous Venom to Overcome Caterpillar Prey
Biology, Vol.12(5), pp.1-17
2023
Abstract
Predatory stink bugs capture prey by injecting salivary venom from their venom glands using specialized stylets. Understanding venom function has been impeded by a scarcity of knowledge of their venom composition. We therefore examined the proteinaceous components of the salivary venom of the predatory stink bug Arma custos (Fabricius, 1794) (Hemiptera: Pentatomidae). We used gland extracts and venoms from fifth-instar nymphs or adult females to perform shotgun proteomics combined with venom gland transcriptomics. We found that the venom of A. custos comprised a complex suite of over a hundred individual proteins, including oxidoreductases, transferases, hydrolases, ligases, protease inhibitors, and recognition, transport and binding proteins. Besides the uncharacterized proteins, hydrolases such as venom serine proteases, cathepsins, phospholipase A2, phosphatases, nucleases, alpha-amylases, and chitinases constitute the most abundant protein families. However, salivary proteins shared by and unique to other predatory heteropterans were not detected in the A. custos venom. Injection of the proteinaceous (>3 kDa) venom fraction of A. custos gland extracts or venom into its prey, the larvae of the oriental armyworm Mythimna separata (Walker, 1865), revealed insecticidal activity against lepidopterans. Our data expand the knowledge of heteropteran salivary proteins and suggest predatory asopine bugs as a novel source for bioinsecticides.
Details
- Title
- The Predatory Stink Bug Arma custos (Hemiptera: Pentatomidae) Produces a Complex Proteinaceous Venom to Overcome Caterpillar Prey
- Authors
- Yuli Qu (Author)Andrew A. Walker (Author) - The University of QueenslandLing Meng (Author)Volker Herzig (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationBaoping Li (Author)
- Publication details
- Biology, Vol.12(5), pp.1-17
- Publisher
- MDPI AG
- Date published
- 2023
- DOI
- 10.3390/biology12050691
- ISSN
- 2079-7737
- Copyright note
- © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).
- Grant note
- 2017YFD0201000/ National Key R&D Program of China 202106850034/ China Scholarship Council DP200102867/ Australian Research Council through Discovery Project CE200100012/ Centre of Excellence
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99728797902621
- Output Type
- Journal article
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