The P450 multigene family of Fontainea and insights into diterpenoid synthesis

Shahida A Mitu; Steven M Ogbourne; Anne H Klein; Trong D Tran; Paul W Reddell; Scott F Cummins

doi:10.1186/s12870-021-02958-y

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The P450 multigene family of Fontainea and insights into diterpenoid synthesis

Journal article

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The P450 multigene family of Fontainea and insights into diterpenoid synthesis

Shahida A Mitu, Steven M Ogbourne, Anne H Klein, Trong D Tran, Paul W Reddell and Scott F Cummins

BMC Plant Biology, Vol.21(1), pp.1-18

2021

DOI: https://doi.org/10.1186/s12870-021-02958-y

PMCID: PMC8058993

PMID: 33879061

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Abstract

Tigilanol tiglate

Biosynthesis

Research

Anti-cancer

EBC-46

Transcriptome-wide identification

Background: Cytochrome P450s (P450s) are enzymes that play critical roles in the biosynthesis of physiologically important compounds across all organisms. Although they have been characterised in a large number of plant species, no information relating to these enzymes are available from the genus Fontainea (family Euphorbiaceae). Fontainea is significant as the genus includes species that produce medicinally significant epoxy-tigliane natural products, one of which has been approved as an anti-cancer therapeutic. Results: A comparative species leaf metabolome analysis showed that Fontainea species possess a chemical profile different from various other plant species. The diversity and expression profiles of Fontainea P450s were investigated from leaf and root tissue. A total of 103 and 123 full-length P450 genes in Fontainea picrosperma and Fontainea venosa, respectively (and a further 127/125 partial-length) that were phylogenetically classified into clans, families and subfamilies. The majority of P450 identified are most active within root tissue (66.2% F. picrosperma, 65.0% F. venosa). Representatives within the CYP71D and CYP726A were identified in Fontainea that are excellent candidates for diterpenoid synthesis, of which CYP726A1, CYP726A2 and CYP71D1 appear to be exclusive to Fontainea species and were significantly more highly expressed in root tissue compared to leaf tissue. Conclusion: This study presents a comprehensive overview of the P450 gene family in Fontainea that may provide important insights into the biosynthesis of the medicinally significant epoxy-tigliane diterpenes found within the genus.

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Title: The P450 multigene family of Fontainea and insights into diterpenoid synthesis
Authors: Shahida A Mitu (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Steven M Ogbourne (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Anne H Klein (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Trong D Tran (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Paul W Reddell (Author) - EcoBiotics Ltd.
Scott F Cummins (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Publication details: BMC Plant Biology, Vol.21(1), pp.1-18
Publisher: BioMed Central Ltd.
Date published: 2021
DOI: 10.1186/s12870-021-02958-y
ISSN: 1471-2229
PMID: 33879061; PMC8058993
Copyright note: © The Author(s). 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99526508302621
Output Type: Journal article

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