The Moraxella adhesin UspA1 binds to its human CEACAM1 receptor by a deformable trimeric coiled-coil

Rebecca Conners; Darryl J Hill; Elena Borodina; Christopher Agnew; Sarah J Daniell; Nicholas M Burton; Richard B Sessions; Anthony R Clarke; Lucy E Catto; Donna Lammie; Tim J Wess; R Leo Brady; Mumtaz Virji

doi:10.1038/emboj.2008.101

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The Moraxella adhesin UspA1 binds to its human CEACAM1 receptor by a deformable trimeric coiled-coil

Journal article

Peer reviewed

The Moraxella adhesin UspA1 binds to its human CEACAM1 receptor by a deformable trimeric coiled-coil

Rebecca Conners, Darryl J Hill, Elena Borodina, Christopher Agnew, Sarah J Daniell, Nicholas M Burton, Richard B Sessions, Anthony R Clarke, Lucy E Catto, Donna Lammie, …

EMBO Journal, Vol.27(12), pp.1779-1789

2008

DOI: https://doi.org/10.1038/emboj.2008.101

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Abstract

adhesion molecules

bacterial adhesin

coiled-coil

SAXS

X-ray crystallography

Moraxella catarrhalis is a ubiquitous human-specific bacterium commonly associated with upper and lower respiratory tract infections, including otitis media, sinusitis and chronic obstructive pulmonary disease. The bacterium uses an autotransporter protein UspA1 to target an important human cellular receptor carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1). Using X-ray crystallography, we show that the CEACAM1 receptor-binding region of UspA1 unusually consists of an extended, rod-like left-handed trimeric coiled-coil. Mutagenesis and binding studies of UspA1 and the N-domain of CEACAM1 have been used to delineate the interacting surfaces between ligand and receptor and guide assembly of the complex. However, solution scattering, molecular modelling and electron microscopy analyses all indicate that significant bending of the UspA1 coiled-coil stalk also occurs. This explains how UspA1 can engage CEACAM1 at a site far distant from its head group, permitting closer proximity of the respective cell surfaces during infection. © 2008 European Molecular Biology Organization | All Rights Reserved.

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Title: The Moraxella adhesin UspA1 binds to its human CEACAM1 receptor by a deformable trimeric coiled-coil
Authors: Rebecca Conners (Author) - University of Bristol, United Kingdom
Darryl J Hill (Author) - University of Bristol, United Kingdom
Elena Borodina (Author) - University of Bristol, United Kingdom
Christopher Agnew (Author) - University of Bristol, United Kingdom
Sarah J Daniell (Author) - University of Bristol, United Kingdom
Nicholas M Burton (Author) - University of Bristol, United Kingdom
Richard B Sessions (Author) - University of Bristol, United Kingdom
Anthony R Clarke (Author) - University of Bristol, United Kingdom
Lucy E Catto (Author) - University of Bristol, United Kingdom
Donna Lammie (Author) - Cardiff University, United Kingdom
Tim J Wess (Author) - Cardiff University, United Kingdom
R Leo Brady (Author) - University of Bristol, United Kingdom
Mumtaz Virji (Author) - University of Bristol, United Kingdom
Publication details: EMBO Journal, Vol.27(12), pp.1779-1789
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2008
DOI: 10.1038/emboj.2008.101
ISSN: 0261-4189
Organisation Unit: Office of the Deputy Vice-Chancellor (Academic); University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450798302621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Cell Biology

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