Journal article
The Kiss2receptor (Kiss2r) gene in Southern Bluefin Tuna, Thunnus maccoyii, and in Yellowtail Kingfish, Seriola lalandi– functional analysis and isolation of transcript variants
Molecular and Cellular Endocrinology, Vol.362(1-2), pp.211-220
2012
Abstract
The kisspeptin system plays an essential role in reproductive function in vertebrates, particularly in the onset of puberty. We investigated the kisspeptin system in two Perciform teleosts, the Southern Bluefin Tuna (SBT; Thunnus maccoyii), and the Yellowtail Kingfish (YTK; Seriola lalandi), by characterising their kisspeptin 2 receptor (Kiss2r) genes. In addition to the full length Kiss2r cDNA sequences, we have isolated from SBT and YTK a transcript variant that retained an intron. We have further obtained three ytkKiss2r transcript variants that contained deletions. In vitro functional analysis of the full length SBT and YTK Kiss2r showed higher response to Kiss2-10 than to Kiss1-10, with stronger transduction via PKC than PKA. The full length ytkKiss2r and two deletion variants were differentially expressed in the brain of male, but not in female, juvenile YTK treated with increasing doses of Kiss2-10 peptide. In the gonads, the expression level of the ytkKiss2r transcripts did not vary significantly either in the male or female fish. This is the first time that transcript variants of the Kiss2r gene that contain deletions and show responsiveness to treatments with kisspeptin have been reported in any teleost.
Details
- Title
- The Kiss2receptor (Kiss2r) gene in Southern Bluefin Tuna, Thunnus maccoyii, and in Yellowtail Kingfish, Seriola lalandi– functional analysis and isolation of transcript variants
- Authors
- Josephine N Nocillado (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringJ Biran (Author) - Hebrew University of Jerusalem, IsraelYing Y Lee (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringB Levavi-Sivan (Author) - Hebrew University of Jerusalem, IsraelAlejandro S Mechaly (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringY Zohar (Author) - University of Maryland, United StatesAbigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Molecular and Cellular Endocrinology, Vol.362(1-2), pp.211-220
- Publisher
- Elsevier Ireland Ltd.
- Date published
- 2012
- DOI
- 10.1016/j.mce.2012.06.024
- ISSN
- 0303-7207
- Copyright note
- Copyright © 2012. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450114202621
- Output Type
- Journal article
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