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Published VersionCC BY V4.0, Open Access
Journal article
The Efficacy of Selected Probiotic Strains and Their Combination to Inhibit the Interaction of Adherent-Invasive Escherichia coli (AIEC) with a Co-Culture of Caco-2:HT29-MTX Cells
Microorganisms, Vol.12(3), pp.1-13
2024
PMID: 38543553
Abstract
The gastrointestinal tract’s microbiota plays a crucial role in human health, with dysbiosis linked to the development of diseases such as inflammatory bowel disease (IBD). Whilst the pathogenic mechanisms underlying IBD remain poorly characterised, adherent-invasive Escherichia coli (AIEC) has been implicated as a microbiological factor in disease pathogenesis. These strains show an enhanced ability to diffusely adhere to and invade intestinal epithelial cells, along with the ability to survive and replicate within macrophages. Probiotics, such as Lactobacillus strains, have been identified as potential treatment options due to their abilities to compete with pathogens for binding sites and regulate the host immune response. In this study, we used four well-characterised Lactobacillus strains and their combination to test their ability to inhibit the adhesion, invasion, and translocation of a well-characterized AIEC strain, F44A-1, in a co-culture of Caco-2 and HT29-MTX cell lines representing the gut epithelium. The results demonstrated that the pre-inoculation of the probiotic candidates 90 min prior to the introduction of the AIEC was more effective in inhibiting AIEC interaction than the co-inoculation of the strains. While the individual probiotic strains greatly reduced AIEC colonisation and invasion of the co-cultured cells, their combination was only more effective in reducing the translocation of the AIEC. These results suggest that probiotics are more effective when used prophylactically against pathogens and that the combination of strains may enhance their efficacy against AIEC translocation once used as a prophylactic measure.
Details
- Title
- The Efficacy of Selected Probiotic Strains and Their Combination to Inhibit the Interaction of Adherent-Invasive Escherichia coli (AIEC) with a Co-Culture of Caco-2:HT29-MTX Cells
- Authors
- Georgia Bradford - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringBehnoush Asgari - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringBronwyn Smit - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringEva Hatje - Queensland University of TechnologyAnna Kuballa - University of the Sunshine Coast, Queensland, School of Health and Behavioural Sciences - LegacyMohammad Katouli (Corresponding Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
- Publication details
- Microorganisms, Vol.12(3), pp.1-13
- Publisher
- MDPI AG
- Date published
- 2024
- DOI
- 10.3390/microorganisms12030502
- ISSN
- 2076-2607
- PMID
- 38543553
- Copyright note
- Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- Data Availability
- Data available upon request.
- Organisation Unit
- School of Health - Biomedicine; School of Science and Engineering - Legacy; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 991011698202621
- Output Type
- Journal article
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