The Arabidopsis thaliana Double-Stranded RNA Binding Proteins DRB1 and DRB2 Are Required for miR160-Mediated Responses to Exogenous Auxin

Kim Zimmerman; Joseph L. Pegler; Jackson M. J. Oultram; David A. Collings; Ming-Bo Wang; Christopher P. L. Grof; Andrew L. Eamens

doi:10.3390/genes15121648

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The Arabidopsis thaliana Double-Stranded RNA Binding Proteins DRB1 and DRB2 Are Required for miR160-Mediated Responses to Exogenous Auxin

Journal article

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The Arabidopsis thaliana Double-Stranded RNA Binding Proteins DRB1 and DRB2 Are Required for miR160-Mediated Responses to Exogenous Auxin

Kim Zimmerman, Joseph L. Pegler, Jackson M. J. Oultram, David A. Collings, Ming-Bo Wang, Christopher P. L. Grof and Andrew L. Eamens

Genes, Vol.15(12), pp.1-26

2024

DOI: https://doi.org/10.3390/genes15121648

PMCID: PMC11675975

PMID: 39766914

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Abstract

Arabidopsis thaliana (Arabidopsis)

root development

microRNA160 (miR160)

miR160 expression module

AUXIN RESPONSE FACTOR10 (ARF10)

ARF16

ARF17

synthetic auxin

DOUBLE-STRANDED RNA BINDING (DRB) proteins DRB1, DRB2, and DRB4 are essential for microRNA (miRNA) production in Arabidopsis thaliana (Arabidopsis) with miR160, and its target genes, AUXIN RESPONSE FACTOR10 (ARF10), ARF16, and ARF17, forming an auxin responsive miRNA expression module crucial for root development. Methods: Wild-type Arabidopsis plants (Columbia-0 (Col-0)) and the drb1, drb2, and drb12 mutants were treated with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D), and the miR160-mediated response of these four Arabidopsis lines was phenotypically and molecularly characterized. Results: In 2,4-D-treated Col-0, drb1 and drb2 plants, altered miR160 abundance and ARF10, ARF16, and ARF17 gene expression were associated with altered root system development. However, miR160-directed molecular responses to treatment with 2,4-D was largely defective in the drb12 double mutant. In addition, via profiling of molecular components of the miR160 expression module in the roots of the drb4, drb14, and drb24 mutants, we uncovered a previously unknown role for DRB4 in regulating miR160 production. Conclusions: The miR160 expression module forms a central component of the molecular and phenotypic response of Arabidopsis plants to exogenous auxin treatment. Furthermore, DRB1, DRB2, and DRB4 are all required in Arabidopsis roots to control miR160 production, and subsequently, to appropriately regulate ARF10, ARF16, and ARF17 target gene expression.

Details

Title: The Arabidopsis thaliana Double-Stranded RNA Binding Proteins DRB1 and DRB2 Are Required for miR160-Mediated Responses to Exogenous Auxin
Authors: Kim Zimmerman - University of Newcastle Australia
Joseph L. Pegler - University of Newcastle Australia
Jackson M. J. Oultram - University of Newcastle Australia
David A. Collings - University of Newcastle Australia
Ming-Bo Wang - CSIRO Agriculture and Food (Australia)
Christopher P. L. Grof - University of Newcastle Australia
Andrew L. Eamens (Corresponding Author) - University of the Sunshine Coast, Queensland, School of Health - Biomedicine
Publication details: Genes, Vol.15(12), pp.1-26
Publisher: MDPI AG
Date published: 2024
DOI: 10.3390/genes15121648
ISSN: 2073-4425
PMID: 39766914; PMC11675975
Copyright note: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Data Availability: The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author.
Organisation Unit: School of Health - Biomedicine
Language: English
Record Identifier: 991091194602621
Output Type: Journal article

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Web Of Science research areas: Genetics & Heredity

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