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Published VersionCC BY V4.0, Open Access
Journal article
Testing the effectiveness of genetic monitoring using genetic non‐invasive sampling
Ecology and Evolution, Vol.12(1), pp.1-12
2021
Abstract
Effective conservation requires accurate data on population genetic diversity, inbreeding, and genetic structure. Increasingly, scientists are adopting genetic non-invasive sampling (gNIS) as a cost-effective population-wide genetic monitoring approach. gNIS has, however, known limitations which may impact the accuracy of downstream genetic analyses. Here, using high-quality single nucleotide polymorphism (SNP) data from blood/tissue sampling of a free-ranging koala population (n = 430), we investigated how the reduced SNP panel size and call rate typical of genetic non-invasive samples (derived from experimental and field trials) impacts the accuracy of genetic measures, and also the effect of sampling intensity on these measures. We found that gNIS at small sample sizes (14% of population) can provide accurate population diversity measures, but slightly underestimated population inbreeding coefficients. Accurate measures of internal relatedness required at least 33% of the population to be sampled. Accurate geographic and genetic spatial autocorrelation analysis requires between 28% and 51% of the population to be sampled. We show that gNIS at low sample sizes can provide a powerful tool to aid conservation decision-making and provide recommendations for researchers looking to apply these techniques to free-ranging systems.
Details
- Title
- Testing the effectiveness of genetic monitoring using genetic non‐invasive sampling
- Authors
- Anthony James Schultz (Corresponding Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyKasha Strickland (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyRomane H Cristescu (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringJonathan Hanger (Author) - Endeavour Veterinary Ecology Pty LtdDeidre Villiers (Author) - Endeavour Veterinary Ecology Pty LtdCéline H Frère (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
- Publication details
- Ecology and Evolution, Vol.12(1), pp.1-12
- Publisher
- John Wiley & Sons Ltd.
- Date published
- 2021
- DOI
- 10.1002/ece3.8459
- ISSN
- 2045-7758
- Copyright note
- © 2021 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99603206002621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Ecology
- Evolutionary Biology