Journal article
Teneurin and TCAP Phylogeny and Physiology: Molecular Analysis, Immune Activity, and Transcriptomic Analysis of the Stress Response in the Sydney Rock Oyster (Saccostrea glomerata) Hemocytes
Frontiers in Endocrinology , Vol.13, pp.1-19
2022
PMCID: PMC9248207
PMID: 35784537
Abstract
Teneurin C-terminal associated peptide (TCAP) is an ancient bioactive peptide that is highly conserved in metazoans. TCAP administration reduces cellular and behavioral stress in vertebrate and urochordate models. There is little information for invertebrates regarding the existence or function of a TCAP. This study used the Sydney rock oyster (SRO) as a molluscan model to characterize an invertebrate TCAP, from molecular gene analysis to its physiological effects associated with hemocyte phagocytosis. We report a single teneurin gene (and 4 teneurin splice variants), which encodes a precursor with TCAP that shares a vertebrate-like motif, and is similar to that of other molluscan classes (gastropod, cephalopod), arthropods and echinoderms. TCAP was identified in all SRO tissues using western blotting at 1-2 different molecular weights (~22 kDa and ~37kDa), supporting precursor cleavage variation. In SRO hemolymph, TCAP was spatially localized to the cytosol of hemocytes, and with particularly high density immunoreactivity in granules. Based on ‘pull-down’ assays, the SRO TCAP binds to GAPDH, suggesting that TCAP may protect cells from apoptosis under oxidative stress. Compared to sham injection, the intramuscular administration of TCAP (5 pmol) into oysters modulated their immune system by significantly reducing hemocyte phagocytosis under stress conditions (low salinity and high temperature). TCAP administration also significantly reduced hemocyte reactive oxygen species production at ambient conditions and after 48 h stress, compared to sham injection. Transcriptomic hemocyte analysis of stressed oysters administered with TCAP demonstrated significant changes in expression of genes associated with key metabolic, protective and immune functions. In summary, this study established a role for TCAP in oysters through modulation of physiological and molecular functions associated with energy conservation, stress and cellular defense.
Details
- Title
- Teneurin and TCAP Phylogeny and Physiology: Molecular Analysis, Immune Activity, and Transcriptomic Analysis of the Stress Response in the Sydney Rock Oyster (Saccostrea glomerata) Hemocytes
- Authors
- Tomer Abramov (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacySaowaros Suwansa-Ard (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyPatricia Mirella da Silva (Author) - Universidade Federal da ParaíbaTianfang Wang (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyMichael Dove (Author) - New South Wales Department of Primary IndustriesWayne O'Connor (Author) - New South Wales Department of Primary IndustriesLaura Parker (Author) - UNSW AustraliaDavid A Lovejoy (Author) - University of TorontoScott F Cummins (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringAbigail Elizur (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
- Publication details
- Frontiers in Endocrinology , Vol.13, pp.1-19
- Publisher
- Frontiers Research Foundation
- Date published
- 2022
- DOI
- 10.3389/fendo.2022.891714
- ISSN
- 1664-2392
- PMID
- 35784537; PMC9248207
- Copyright note
- © 2022 Abramov, Suwansa-ard, da Silva, Wang, Dove, O’Connor, Parker, Lovejoy, Cummins and Elizur. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Data Availability
- The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://www.ncbi. nlm.nih.gov/, PRJNA804582.
- Grants
- Future Oysters CRC-P, 0980024282, Cooperative Research Centres
- Organisation Unit
- GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99654998702621
- Output Type
- Journal article
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