Temporal profile of β follicle-stimulating hormone, β luteinizing hormone, and growth hormone gene expression in the protandrous hermaphrodite, gilthead seabream, Sparus aurata

I Meiri; Wayne R Knibb; Y Zohar; Abigail Elizur

doi:10.1016/j.ygcen.2004.04.002

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Temporal profile of β follicle-stimulating hormone, β luteinizing hormone, and growth hormone gene expression in the protandrous hermaphrodite, gilthead seabream, Sparus aurata

I Meiri, Wayne R Knibb, Y Zohar and Abigail Elizur

General and Comparative Endocrinology, Vol.137(3), pp.288-299

2004

DOI: https://doi.org/10.1016/j.ygcen.2004.04.002

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Abstract

Clinical Sciences

gonadotropins

βFSH

βLH

seabream

mRNA

gene expression

ontogeny of gene expression

The temporal profile of βFSH, βLH, and growth hormone (GH) gene expression was measured throughout the periods of gonadal development, spawning, and post-spawning in the protandrous hermaphrodite gilthead seabream (sb), Sparus aurata (L.). Sampling was carried out monthly, covering a 8-31 month fish age. Pituitary RNA was extracted individually. The levels of βFSH, βLH, and GH mRNA were measured by dot blot hybridization using sb βFSH, βLH, and GH cDNA as probes and analyzed by computing densitometer (values standardized using individuals' β-actin pituitary mRNA levels). All three genes, βFSH, βLH, and GH were expressed throughout the year, with seasonal variations. However, transcript levels of βLH were consistently higher than those of βFSH. During the spawning season (which lasts for about 4 months), the mRNA levels of both βFSH and βLH subunits increased dramatically. βFSH peaked at the start of the spawning season for both males and females, and was significantly higher in males. As for βLH transcripts, a statistical interaction between sex and date was observed. No significant differences between males and females were found for GH. The pattern of GH expression levels was found to be correlated to that of βLH.

Details

Title: Temporal profile of β follicle-stimulating hormone, β luteinizing hormone, and growth hormone gene expression in the protandrous hermaphrodite, gilthead seabream, Sparus aurata
Authors: I Meiri (Author) - Israel Oceanographic and Limnological Research, Israel
Wayne R Knibb (Author) - Israel Oceanographic and Limnological Research, Israel
Y Zohar (Author) - University of Maryland, United States
Abigail Elizur (Author) - Israel Oceanographic and Limnological Research, Israel
Publication details: General and Comparative Endocrinology, Vol.137(3), pp.288-299
Publisher: Academic Press
Date published: 2004
DOI: 10.1016/j.ygcen.2004.04.002
ISSN: 0016-6480
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449364302621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Endocrinology & Metabolism; Zoology

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