Temporal expression of G-protein-coupled receptor 54 (GPR54), gonadotropin-releasing hormones (GnRH), and dopamine receptor D2 (drd2) in pubertal female grey mullet, Mugil cephalus

Josephine N Nocillado; B Levavi-Sivan; F Carrick; Abigail Elizur

doi:10.1016/j.ygcen.2006.09.008

Back

Temporal expression of G-protein-coupled receptor 54 (GPR54), gonadotropin-releasing hormones (GnRH), and dopamine receptor D2 (drd2) in pubertal female grey mullet, Mugil cephalus

Journal article

Peer reviewed

Temporal expression of G-protein-coupled receptor 54 (GPR54), gonadotropin-releasing hormones (GnRH), and dopamine receptor D2 (drd2) in pubertal female grey mullet, Mugil cephalus

Josephine N Nocillado, B Levavi-Sivan, F Carrick and Abigail Elizur

General and Comparative Endocrinology, Vol.150(2), pp.278-287

2007

DOI: https://doi.org/10.1016/j.ygcen.2006.09.008

Files and links (1)

url

https://doi.org/10.1016/j.ygcen.2006.09.008View

Published Version

Abstract

puberty

GPR54

GnRH

dopamine receptor

brain-pituitary-gonadal axis

mullet

The G-protein-coupled receptor 54 (muGPR54) cDNA was cloned from the brain of the grey mullet, and its expression level, as well as those of the gonadotropin-releasing hormones (GnRH1, GnRH2, GnRH3) and dopamine receptor D2 (drd2), in the brain, pituitary and ovary of pubertal fish (early, intermediate, advanced) were determined by real-time quantitative RT-PCR (QPCR). The muGPR54 cDNA has an open reading frame of 1140 bp with a predicted 380 amino acid peptide, containing seven putative transmembrane domains and putative N-glycosylation and protein kinase C phosphorylation sites. QPCR results showed that the early stage of puberty in grey mullet is characterized by significantly high levels of expression of GPR54, GnRH and drd2 in the brain relative to the intermediate and advanced stages, except for GnRH1 that increased at the advanced stage of puberty. In the pituitary, drd2 expression declined significantly at the advanced stage relative to levels at the intermediate stage. Ovarian expression of GPR54 significantly increased from the intermediate stage of puberty relative to the early stage while that of GnRH1 acutely increased at the advanced stage of puberty. The ovarian expression of drd2 decreased as puberty progressed, but the changes were not significant. The results suggest the possible role of GPR54 and GnRH in positively regulating pubertal development in grey mullet and the dopaminergic inhibition of reproductive function mediated by drd2.

Details

Title: Temporal expression of G-protein-coupled receptor 54 (GPR54), gonadotropin-releasing hormones (GnRH), and dopamine receptor D2 (drd2) in pubertal female grey mullet, Mugil cephalus
Authors: Josephine N Nocillado (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
B Levavi-Sivan (Author) - Hebrew University of Jerusalem
F Carrick (Author) - University of Queensland
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Publication details: General and Comparative Endocrinology, Vol.150(2), pp.278-287
Publisher: Academic Press
Date published: 2007
DOI: 10.1016/j.ygcen.2006.09.008
ISSN: 0016-6480
Organisation Unit: University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449198402621
Output Type: Journal article

Metrics

2 File views/ downloads

811 Record Views

105 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Endocrinology & Metabolism; Zoology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites