Temporal Silencing of an Androgenic Gland-Specific Insulin-Like Gene Affecting Phenotypical Gender Differences and Spermatogenesis

Tomer Ventura; R Manor; E D Aflalo; S Weil; L Glazer; A Sagi

doi:10.1210/en.2008-0906

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Temporal Silencing of an Androgenic Gland-Specific Insulin-Like Gene Affecting Phenotypical Gender Differences and Spermatogenesis

Journal article

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Temporal Silencing of an Androgenic Gland-Specific Insulin-Like Gene Affecting Phenotypical Gender Differences and Spermatogenesis

Tomer Ventura, R Manor, E D Aflalo, S Weil, L Glazer and A Sagi

Endocrinology, Vol.150(3), p.1278–1286

2009

DOI: https://doi.org/10.1210/en.2008-0906

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Abstract

Androgenic glands (AGs) of the freshwater prawn Macrobrachium rosenbergii were subjected to endocrine manipulation, causing them to hypertrophy. Transcripts from these glands were used in the construction of anAGcDNAsubtractive library. Screening of the library revealed an AG-specific gene, termed the M. rosenbergii insulin-like AG (Mr-IAG) gene. The cDNA of this gene was then cloned and fully sequenced. The cysteine backbone of the predicted mature Mr-IAG peptide (B and Achains)showedhigh similarity to that of other crustacean AG-specific insulin-like peptides. In vivo silencing of the gene, by injecting the prawns with Mr-IAG double-stranded RNA, temporarily prevented the regeneration of male secondary sexual characteristics, accompanied by a lag in molt and a reduction in growth parameters, which are typically higher in males of the species. In terms of reproductive parameters, silencing of Mr-IAG led to the arrest of testicular spermatogenesis and of spermatophore development in the terminal ampullae of the sperm duct, accompanied by hypertrophy and hyperplasia of the AGs. This study constitutes the first report of the silencing of a gene expressed specifically in the AG, which caused a transient adverse effect on male phenotypical gender differences and spermatogenesis.

Details

Title: Temporal Silencing of an Androgenic Gland-Specific Insulin-Like Gene Affecting Phenotypical Gender Differences and Spermatogenesis
Authors: Tomer Ventura (Author) - Ben-Gurion University of the Negev, Israel
R Manor (Author) - Ben-Gurion University of the Negev, Israel
E D Aflalo (Author) - Ben-Gurion University of the Negev, Israel
S Weil (Author) - Ben-Gurion University of the Negev, Israel
L Glazer (Author) - Ben-Gurion University of the Negev, Israel
A Sagi (Author) - Ben-Gurion University of the Negev, Israel
Publication details: Endocrinology, Vol.150(3), p.1278–1286
Publisher: Endocrine Society
Date published: 2009
DOI: 10.1210/en.2008-0906
ISSN: 0013-7227
Copyright note: Copyright © 2009 by The Endocrine Society. Reproduced with permission of the copyright holder.
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450121302621
Output Type: Journal article

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