TRPV3 expression and vasodilator function in isolated uterine radial arteries from non-pregnant and pregnant rats

T V Murphy; A Kanagarajah; S Toemoe; P P Bertrand; T Hilton Grayson; Fiona C Britton; Leo Leader; S Senadheera; Shaun L Sandow

doi:10.1016/j.vph.2016.04.004

Back

TRPV3 expression and vasodilator function in isolated uterine radial arteries from non-pregnant and pregnant rats

Journal article

Peer reviewed

TRPV3 expression and vasodilator function in isolated uterine radial arteries from non-pregnant and pregnant rats

T V Murphy, A Kanagarajah, S Toemoe, P P Bertrand, T Hilton Grayson, Fiona C Britton, Leo Leader, S Senadheera and Shaun L Sandow

Vascular Pharmacology, Vol.83, pp.66-77

2016

DOI: https://doi.org/10.1016/j.vph.2016.04.004

Files and links (1)

url

https://doi.org/10.1016/j.vph.2016.04.004View

Published Version

Abstract

TRPV3

carvacrol

uterus

radial artery

pregnancy

This study investigated the expression and function of transient receptor potential vanilloid type-3 ion channels (TRPV3) in uterine radial arteries isolated from non-pregnant and twenty-day pregnant rats. Immunohistochemistry (IHC) suggested TRPV3 is primarily localized to the smooth muscle in arteries from both non-pregnant and pregnant rats. IHC using Câ€² targeted antibody, and qPCR of TRPV3 mRNA, suggested pregnancy increased arterial TRPV3 expression. The TRPV3 activator carvacrol caused endothelium-independent dilation of phenylephrine-constricted radial arteries, with no difference between vessels from non-pregnant and pregnant animals. Carvacrol-induced dilation was reduced by the TRPV3-blockers isopentenyl pyrophosphate and ruthenium red, but not by the TRPA1 or TRPV4 inhibitors HC-030031 or HC-067047, respectively. In radial arteries from non-pregnant rats only, inhibition of NOS and sGC, or PKG, enhanced carvacrol-mediated vasodilation. Carvacrol-induced dilation of arteries from both non-pregnant and pregnant rats was prevented by the IKCa blocker TRAM-34. TRPV3 caused an endothelium-independent, IKCa-mediated dilation of the uterine radial artery. NO-PKG-mediated modulation of TRPV3 activity is lost in pregnancy, but this did not alter the response to carvacrol.

Details

Title: TRPV3 expression and vasodilator function in isolated uterine radial arteries from non-pregnant and pregnant rats
Authors: T V Murphy (Author) - University of New South Wales
A Kanagarajah (Author) - University of New South Wales
S Toemoe (Author) - University of New South Wales
P P Bertrand (Author) - University of New South Wales
T Hilton Grayson (Author) - University of New South Wales
Fiona C Britton (Author) - University of New South Wales
Leo Leader (Author) - University of New South Wales
S Senadheera (Author) - University of New South Wales
Shaun L Sandow (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Vascular Pharmacology, Vol.83, pp.66-77
Publisher: Elsevier Inc.
Date published: 2016
DOI: 10.1016/j.vph.2016.04.004
ISSN: 1537-1891
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450072802621
Output Type: Journal article

Metrics

8 File views/ downloads

776 Record Views

24 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Web Of Science research areas: Pharmacology & Pharmacy

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites