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Synthetic seed propagation of the therapeutic-honey plants Leptospermum polygalifolium and L. scoparium (Myrtaceae)1.58 MB
Published VersionCC BY-NC-ND V4.0, Open Access
Journal article
Synthetic seed propagation of the therapeutic-honey plants Leptospermum polygalifolium and L. scoparium (Myrtaceae)
Australian Journal of Botany, Vol.70(6), pp.447-454
2022
Abstract
Context: Increasing demand for therapeutic honey has driven establishment of Leptospermum nectar plantations. Methods for propagation involving synthetic seeds (artificially encapsulated miniature cuttings) may speed production of Leptospermum polygalifolium Salisb. and L. scoparium J.R.Forst. & G.Forst. Aims: The study aimed to determine how nutrient strength of the encapsulation solution and the presence of benzyladenine (BA) in the emergence medium affect shoot and root emergence from synthetic seeds of L. polygalifolium and L. scoparium. Methods: Nodes from in vitro shoots of three L. polygalifolium clones (P1, P6, P11) and two L. scoparium clones (S6, S12) were encapsulated in 3% sodium alginate with half or full strength Murashige and Skoog (MS) medium, and the synthetic seeds were placed on full strength MS emergence medium containing 0 or 2.2 μM BA. Key results: Full strength MS in the encapsulation solution was effective for shoot emergence of both species. BA increased the percentage of synthetic seeds with shoot emergence in clone P6 but decreased the percentage in clone S12. BA stimulated shoot emergence through callus in clones P1, P6, S6 and S12, and increased the number of shoots per emergent synthetic seed in clones S6 and S12. Surprisingly, the simple use of full strength MS medium without hormones was highly effective for adventitious rooting, stimulating root emergence and plantlet formation in 26 to 57% of L. polygalifolium and 100% of L. scoparium synthetic seeds. Conclusions: These two Leptospermum species are highly amenable to propagation via synthetic seeds. A simple formulation of hormone free, full strength MS medium in the encapsulation solution and emergence medium provides high frequencies of plantlet conversion. Implications: Synthetic seeds have potential to assist in mass production of Leptospermum plants for nectar plantations to meet demand for therapeutic honey.
Details
- Title
- Synthetic seed propagation of the therapeutic-honey plants Leptospermum polygalifolium and L. scoparium (Myrtaceae)
- Authors
- Ian Darby (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationAaron Wiegand (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationShahla Hosseini Bai (Author) - Griffith UniversityHelen Wallace (Author) - Griffith UniversityStephen Trueman (Corresponding Author) - Griffith University
- Publication details
- Australian Journal of Botany, Vol.70(6), pp.447-454
- Publisher
- C S I R O Publishing
- Date published
- 2022
- DOI
- 10.1071/BT22025
- ISSN
- 1444-9862; 0067-1924
- Copyright note
- © 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons AttributionNonCommercial NoDerivatives 4.0 International License (CC BY-NC-ND).
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Student Services and Engagement; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99681397402621
- Output Type
- Journal article
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