Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site

Xuan Pan; Bowei Ma; Xinchao You; Shu Chen; Jialing Wu; Tianfang Wang; Shelley F Walton; Jianwei Yuan; Xiaolian Wu; Guoqiang Chen; Yuejian Wang; Guoying Ni; Xiao Song Liu

doi:10.1186/s12906-019-2571-z

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Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site

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Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site

Xuan Pan, Bowei Ma, Xinchao You, Shu Chen, Jialing Wu, Tianfang Wang, Shelley F Walton, Jianwei Yuan, Xiaolian Wu, Guoqiang Chen, …

BMC Complementary and Alternative Medicine, Vol.19, 163

2019

DOI: https://doi.org/10.1186/s12906-019-2571-z

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Abstract

caerin peptide

therapeutic vaccine

cervical cancer

tumour microenvironment

Background: Therapeutic vaccines against cervical cancer remain ineffective. Previously, we demonstrated that blocking the signalling of a cytokine, interleukin 10, at the time of immunisation elicited significantly higher numbers of antigen specific T cells and inhibited tumour growth in mice. Results: In the current paper, we demonstrate, in a HPV16 E6/E7 transformed TC-1 tumour mouse model, that despite increased antigen specific T cell numbers, blocking IL-10 signalling at the time of immunisation does not increase the survival time of the TC-1 tumour bearing mice compared to mice receiving the same immunisation with no IL-10 signalling blockade. Moreover, the function of tumour infiltrating T cells isolated 3 weeks post TC-1 transplantation is more suppressed than those isolated 2 weeks after tumour inoculation. We demonstrate that synthesized caerin peptides, derived from amphibian skin secretions, 1) were able to inhibit TC-1 tumour growth both in vitro and in vivo; 2) are environmentally stable; and 3) promote the secretion of pro-inflammatory interlukine-6 by TC-1 cells. Notably caerin peptides were able to increase the survival time of TC-1 tumour bearing mice after therapeutic vaccination with a HPV16E7 peptide-based vaccine containing IL-10 inhibitor, via recruiting increased levels of T cells to the tumour site. Conclusion: Caerin peptides increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site.

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Title: Synthesized natural peptides from amphibian skin secretions increase the efficacy of a therapeutic vaccine by recruiting more T cells to the tumour site
Authors: Xuan Pan (Author) - Guangdong Pharmaceutical University, China
Bowei Ma (Author) - Guangdong Pharmaceutical University, China
Xinchao You (Author) - Guangdong Pharmaceutical University, China
Shu Chen (Author) - Foshan First People's Hospital, China
Jialing Wu (Author) - Foshan First People's Hospital, China
Tianfang Wang (Author) - University of the Sunshine Coast
Shelley F Walton (Author) - University of the Sunshine Coast
Jianwei Yuan (Author) - Guangdong Pharmaceutical University, China
Xiaolian Wu (Author) - Foshan First People's Hospital, China
Guoqiang Chen (Author) - Foshan First People's Hospital, China
Yuejian Wang (Author) - Foshan First People's Hospital, China
Guoying Ni (Author) - University of the Sunshine Coast
Xiao Song Liu (Author) - University of the Sunshine Coast
Publication details: BMC Complementary and Alternative Medicine, Vol.19, 163; 13
Publisher: BioMed Central Ltd.
Date published: 2019
DOI: 10.1186/s12906-019-2571-z
ISSN: 1472-6882
Copyright note: Copyright © 2019 The Authors. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450623102621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Integrative & Complementary Medicine

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