Journal article
Synphilin-1 and parkin show overlapping expression patterns in human brain and form aggresomes in response to proteasomal inhibition
Neurobiology of Disease, Vol.20(2), pp.401-411
2005
Abstract
Lewy bodies (LBs) are the characteristic inclusions of Parkinson's disease brain but the mechanism responsible for their formation is obscure. Lewy bodies (LBs) are composed of a number of proteins of which alpha-synuclein (α-SYN) is a major constituent. In this study, we have investigated the distribution patterns of synphilin-1 and parkin proteins in control and sporadic PD brain tissue by immunohistochemistry (IH), immunoblotting, and immunoelectron microscopy (IEM). We demonstrate the presence of synphilin-1 and parkin in the central core of a majority of LBs using IH and IEM. Using IH, we show an overlapping distribution profile of the two proteins in central neurons. Additionally, we show sensitivity of both endogenous synphilin-1 and parkin to proteolytic dysfunction and their co-localization in aggresomes formed in response to the proteasome inhibitor MG-132. We confirm that synphilin-1 and parkin are components of majority of LBs in Parkinson's disease and that both proteins are susceptible to proteasomal degradation. © 2005 Elsevier Inc. All rights reserved.
Details
- Title
- Synphilin-1 and parkin show overlapping expression patterns in human brain and form aggresomes in response to proteasomal inhibition
- Authors
- R Bandopadhyay (Author) - Reta Lila Weston Institute of Neurological Studies, United KingdomA E Kingsbury (Author) - Reta Lila Weston Institute of Neurological Studies, United KingdomM M Muqit (Author) - Institute of Child Health, United KingdomK Harvey (Author) - University College London, United KingdomA R Reid (Author) - Reta Lila Weston Institute of Neurological Studies, United KingdomL Kilford (Author) - Institute of Neurology, United KingdomS Engelender (Author) - Technion Israel-Institute of Technology, IsraelM G Schlossmacher (Author) - Centre for Neurologic Diseases, United StatesN W Wood (Author) - Institute of Neurology, United KingdomD S Latchman (Author) - Institute of Child Health, United KingdomRobert J Harvey (Author) - University College London, United KingdomA J Lees (Author) - Reta Lila Weston Institute of Neurological Studies, United Kingdom
- Publication details
- Neurobiology of Disease, Vol.20(2), pp.401-411
- Publisher
- Academic Press
- DOI
- 10.1016/j.nbd.2005.03.021
- ISSN
- 0969-9961
- Organisation Unit
- Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health
- Language
- English
- Record Identifier
- 99450914602621
- Output Type
- Journal article
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