SynArfGEF is a guanine nucleotide exchange factor for Arf6 and localizes preferentially at post-synaptic specializations of inhibitory synapses

M Fukaya; A Kamata; Y Hara; H Tamaki; O Katsumata; N Ito; S Takeda; Y Hata; T Suzuki; M Watanabe; Robert J Harvey; H Sakagami

doi:10.1111/j.1471-4159.2010.07167.x

Back

SynArfGEF is a guanine nucleotide exchange factor for Arf6 and localizes preferentially at post-synaptic specializations of inhibitory synapses

Journal article

Peer reviewed

SynArfGEF is a guanine nucleotide exchange factor for Arf6 and localizes preferentially at post-synaptic specializations of inhibitory synapses

M Fukaya, A Kamata, Y Hara, H Tamaki, O Katsumata, N Ito, S Takeda, Y Hata, T Suzuki, M Watanabe, …

Journal of Neurochemistry, Vol.116(6), pp.1122-1137

2011

DOI: https://doi.org/10.1111/j.1471-4159.2010.07167.x

Files and links (1)

url

https://doi.org/10.1111/j.1471-4159.2010.07167.xView

Published Version

Abstract

ADP-ribosylation factor 6

dystrophin

gephyrin

PDZ domain

post-synaptic density

SynArfGEF, also known as BRAG3 or IQSEC3, is a member of the brefeldin A-resistant Arf-GEF/IQSEC family and was originally identified by screening for mRNA species associated with the post-synaptic density fraction. In this study, we demonstrate that synArfGEF activates Arf6, using Arf pull down and transferrin incorporation assays. Immunohistochemical analysis reveals that synArfGEF is present in somata and dendrites as puncta in close association with inhibitory synapses, whereas immunoelectron microscopic analysis reveals that synArfGEF localizes preferentially at post-synaptic specializations of symmetric synapses. Using yeast two-hybrid and pull down assays, we show that synArfGEF is able to bind utrophin/dystrophin and S-SCAM/MAGI-2 scaffolding proteins that localize at inhibitory synapses. Double immunostaining reveals that synArfGEF co-localizes with dystrophin and S-SCAM in cultured hippocampal neurons and cerebellar cortex, respectively. Both β-dystroglycan and S-SCAM were immunoprecipitated from brain lysates using anti-synArfGEF IgG. Taken together, these findings suggest that synArfGEF functions as a novel regulator of Arf6 at inhibitory synapses and associates with the dystrophin-associated glycoprotein complex and S-SCAM. © 2011 International Society for Neurochemistry.

Details

Title: SynArfGEF is a guanine nucleotide exchange factor for Arf6 and localizes preferentially at post-synaptic specializations of inhibitory synapses
Authors: M Fukaya (Author) - Kitasato University School of Medicine, Japan
A Kamata (Author) - Kitasato University School of Medicine, Japan
Y Hara (Author) - Kitasato University School of Medicine, Japan
H Tamaki (Author) - Kitasato University School of Medicine, Japan
O Katsumata (Author) - Kitasato University School of Medicine, Japan
N Ito (Author) - National Center of Neurology and Psychiatry, Japan
S Takeda (Author) - National Center of Neurology and Psychiatry, Japan
Y Hata (Author) - Tokyo Medical and Dental University, Japan
T Suzuki (Author) - Shinshu University Graduate School of Medicine, Japan
M Watanabe (Author) - Hokkaido University School of Medicine, Japan
Robert J Harvey (Author) - University College London, United Kingdom
H Sakagami (Author) - Kitasato University School of Medicine, Japan
Publication details: Journal of Neurochemistry, Vol.116(6), pp.1122-1137
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2011
DOI: 10.1111/j.1471-4159.2010.07167.x
ISSN: 0022-3042
Organisation Unit: School of Health; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450524502621
Output Type: Journal article

Metrics

359 Record Views

48 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Neurosciences

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites