Substrate Specificity and Expression of Three 2,3-Dihydroxybiphenyl 1,2-Dioxygenases from Rhodococcus globerulus Strain P6

David B McKay; M Prucha; W Reineke; K N Timmis; D H Pieper

doi:10.1128/JB.185.9.2944-2951.2003

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Substrate Specificity and Expression of Three 2,3-Dihydroxybiphenyl 1,2-Dioxygenases from Rhodococcus globerulus Strain P6

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Substrate Specificity and Expression of Three 2,3-Dihydroxybiphenyl 1,2-Dioxygenases from Rhodococcus globerulus Strain P6

David B McKay, M Prucha, W Reineke, K N Timmis and D H Pieper

Journal of Bacteriology, Vol.185(9), pp.2944-2951

2003

DOI: https://doi.org/10.1128/JB.185.9.2944-2951.2003

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Abstract

Rhodococcus globerulus

Rhodococcus globerulus strain P6 contains at least three genes, bphC1, bphC2, and bphC3, coding for 2,3-dihydroxybiphenyl 1,2-dioxygenases; the latter two specify enzymes of the family of one-domain extradiol dioxygenases. In order to assess the importance of these different isoenzymes for the broad catabolic activity of this organism towards the degradation of polychlorinated biphenyls (PCBs), the capacities of recombinant enzymes expressed in Escherichia coli to transform different chlorosubstituted dihydroxybiphenyls formed by the action of R. globerulus P6 biphenyl dioxygenase and biphenyl 2,3-dihydrodiol dehydrogenase were determined. Whereas both BphC2 and BphC3 showed similar activities for 2,3-dihydroxybiphenyl and all monochlorinated 2,3-dihydroxybiphenyls, BphC1 exhibited only weak activity for 2'-chloro-2,3-dihydroxybiphenyl. More highly chlorinated 2'-chlorosubstituted 2,3-dihydroxybiphenyls were also transformed at high rates by BphC2 and BphC3 but not BphC1. In R. globerulus P6, BphC2 was constitutively expressed, BphC1 expression was induced during growth on biphenyl, and BphC3 was not expressed at significant levels under the experimental conditions. Although we cannot rule out the expression of BphC3 under certain environmental conditions, it seems that the contrasting substrate specificities of BphC1 and BphC2 contribute significantly to the versatile PCB-degrading phenotype of R. globerulus P6.

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Title: Substrate Specificity and Expression of Three 2,3-Dihydroxybiphenyl 1,2-Dioxygenases from Rhodococcus globerulus Strain P6
Authors: David B McKay (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
M Prucha (Author) - German Research Centre for Biotechnology, Germany
W Reineke (Author) - Bergische Universitat Wuppertal, Germany
K N Timmis (Author) - German Research Centre for Biotechnology, Germany
D H Pieper (Author) - German Research Centre for Biotechnology, Germany
Publication details: Journal of Bacteriology, Vol.185(9), pp.2944-2951
Publisher: American Society for Microbiology
Date published: 2003
DOI: 10.1128/JB.185.9.2944-2951.2003
ISSN: 0021-9193
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99447756602621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Microbiology