Structure of type I and type III heterotypic collagen fibrils: An X-ray diffraction study

G J Cameron; I L Alberts; J H Laing; Tim J Wess

doi:10.1006/jsbi.2002.4459

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$Structure of type I and type III heterotypic collagen fibrils: An X-ray diffraction study$

Journal article

Peer reviewed

Structure of type I and type III heterotypic collagen fibrils: An X-ray diffraction study

G J Cameron, I L Alberts, J H Laing and Tim J Wess

Journal of Structural Biology, Vol.137(1-2), pp.15-22

2002

DOI: https://doi.org/10.1006/jsbi.2002.4459

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Abstract

colonic submucosa

type I/III collagen

heterotypic fibrils

helicoidal molecular packing

molecular modeling

X-ray diffraction

The molecular packing arrangement within collagen fibrils has a significant effect on the tensile properties of tissues. To date, most studies have focused on homotypic fibrils composed of type I collagen. This study investigates the packing of type I/III collagen molecules in heterotypic fibrils of colonic submucosa using a combination of X-ray diffraction data, molecular model building, and simulated X-ray diffraction fibre diagrams. A model comprising a 70-nm-diameter D-(∼65 nm) axial periodic structure containing type I and type III collagen chains was constructed from amino acid scattering factors organised in a liquid-like lateral packing arrangement simulated using a classical Lennard-Jones potential. The models that gave the most accurate correspondence with diffraction data revealed that the structure of the fibril involves liquid-like lateral packing combined with a constant helical inclination angle for molecules throughout the fibril. Combinations of type I:type III scattering factors in a ratio of 4:1 gave a reasonable correspondence with the meridional diffraction series. The attenuation of the meridional intensities may be explained by a blurring of the electron density profile of the D period caused by nonspecific or random interactions between collagen types I and III in the heterotypic fibril. © 2002 Elsevier Science (USA).

Details

Title: Structure of type I and type III heterotypic collagen fibrils: An X-ray diffraction study
Authors: G J Cameron (Author) - University of Stirling, United Kingdom
I L Alberts (Author) - University of Stirling, United Kingdom
J H Laing (Author) - University of Stirling, United Kingdom
Tim J Wess (Author) - University of Stirling, United Kingdom
Publication details: Journal of Structural Biology, Vol.137(1-2), pp.15-22
Publisher: Academic Press
Date published: 2002
DOI: 10.1006/jsbi.2002.4459
ISSN: 1047-8477
Organisation Unit: Office of the Deputy Vice-Chancellor (Academic); University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450665302621
Output Type: Journal article

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Web Of Science research areas: Biochemistry & Molecular Biology; Biophysics; Cell Biology