Structure and pharmacological properties of a molluscan glutamate-gated cation channel and its likely role in feeding behavior

T Stühmer; M Amar; Robert J Harvey; I Bermudez; J Van Minnen; M G Darlison

doi:10.1523/JNEUROSCI.16-09-02869.1996

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Structure and pharmacological properties of a molluscan glutamate-gated cation channel and its likely role in feeding behavior

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Structure and pharmacological properties of a molluscan glutamate-gated cation channel and its likely role in feeding behavior

T Stühmer, M Amar, Robert J Harvey, I Bermudez, J Van Minnen and M G Darlison

Journal of Neuroscience, Vol.16(9), pp.2869-2880

1996

DOI: https://doi.org/10.1523/JNEUROSCI.16-09-02869.1996

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Abstract

4-cluster motorneurons

buccal ganglion

complementary DNA cloning

feeding behavior

in situ hybridization

ion channel

ionotropic glutamate receptor

kainate receptor

mollusc (Lymnaea stagnalis)

xenopus oocyte expression

We describe the isolation of a molluscan (Lymnaea stagnalis) full-length complementary DNA that encodes a mature polypeptide (which we have named Lym- eGluR2) with a predicted molecular weight of 105 kDa that exhibits 44-48% identity to the mammalian kainate-selective glutamate receptor GluR5, GluR6, and GluR7 subunits. Injection of in vitro-transcribed RNA from this clone into Xenopus laevis oocytes results in the robust expression of homo- oligomeric cation channels that can be gated by L-glutamate (EC50 = 1.2±0.3 μM) and several other glutamate receptor agonists; rank order of potency: glutamate >> kainate > ibotenate > AMPA. These currents can be blocked by the mammalian non-NMDA receptor antagonists 6,7- dinitroquinoxaline-2,3-dione, 6-cyano-7-nitroquinoxaline-2,3-dione, and 1- (4-chlorobenzoyl)piperazine-2,3-dicarboxylic acid. Ionic-replacement experiments have shown that the agonist-induced current is carried entirely by sodium and potassium ions. In situ hybridization has revealed that the Lym-eGluR2 transcript is present in all 11 ganglia of the Lymnaea CNS, including the 4-cluster motorneurons within the paired buccal ganglia. The pharmacological properties and deduced location of Lym-eGluR2 are entirely consistent with it being (a component of) the receptor, which has been identified previously on buccal motorneurons, that mediates the excitatory effects of glutamate released from neurons within the feeding central pattern generator.

Details

Title: Structure and pharmacological properties of a molluscan glutamate-gated cation channel and its likely role in feeding behavior
Authors: T Stühmer (Author) - Universität Hamburg, Germany
M Amar (Author) - Universität Hamburg, Germany
Robert J Harvey (Author) - Universität Hamburg, Germany
I Bermudez (Author) - Oxford Brookes University, United Kingdom
J Van Minnen (Author) - Vrije Universiteit, Netherlands
M G Darlison (Author) - Universität Hamburg, Germany
Publication details: Journal of Neuroscience, Vol.16(9), pp.2869-2880
Publisher: Society for Neuroscience (SFN)
Date published: 1996
DOI: 10.1523/JNEUROSCI.16-09-02869.1996
ISSN: 0270-6474
Copyright note: Copyright © 1996 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Organisation Unit: School of Health; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450915602621
Output Type: Journal article

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