Journal article
Structural venomics reveals evolution of a complex venom by duplication and diversification of an ancient peptide-encoding gene
Proceedings of the National Academy of Sciences - PNAS, Vol.117(21), pp.11399-11408
2020
PMID: 32398368
Abstract
Spiders are one of the most successful venomous animals, with more than 48,000 described species. Most spider venoms are dominated by cysteine-rich peptides with a diverse range of pharmacological activities. Some spider venoms contain thousands of unique peptides, but little is known about the mechanisms used to generate such complex chemical arsenals. We used an integrated transcriptomic, proteomic, and structural biology approach to demonstrate that the lethal Australian funnel-web spider produces 33 superfamilies of venom peptides and proteins. Twenty-six of the 33 superfamilies are disulfide-rich peptides, and we show that 15 of these are knottins that contribute >90% of the venom proteome. NMR analyses revealed that most of these disulfide-rich peptides are structurally related and range in complexity from simple to highly elaborated knottin domains, as well as double-knot toxins, that likely evolved from a single ancestral toxin gene.
Details
- Title
- Structural venomics reveals evolution of a complex venom by duplication and diversification of an ancient peptide-encoding gene
- Authors
- Sandy S Pineda (Author) - University of QueenslandYanni K.-Y Chin (Author) - University of QueenslandEivind A. B Undheim (Author) - University of QueenslandSebastian Senff (Author) - University of QueenslandMehdi Mobli (Author) - University of QueenslandClaire Dauly (Author) - Thermo Fisher ScientificPierre Escoubas (Author) - Nice Sophia Antipolis UniversityGraham M Nicholson (Author) - University of Technology SydneyQuentin Kaas (Author) - University of QueenslandShaodong Guo (Author) - University of QueenslandVolker Herzig (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyJohn S Mattick (Author) - Garvan Institute of Medical ResearchGlenn F King (Author) - University of Queensland
- Publication details
- Proceedings of the National Academy of Sciences - PNAS, Vol.117(21), pp.11399-11408
- Publisher
- National Academy of Sciences
- DOI
- 10.1073/pnas.1914536117
- ISSN
- 1091-6490
- PMID
- 32398368
- Organisation Unit
- Centre for Bioinnovation; School of Science, Technology and Engineering; University of the Sunshine Coast, Queensland; School of Science and Engineering - Legacy
- Language
- English
- Record Identifier
- 99482307402621
- Output Type
- Journal article
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- Biochemistry & Molecular Biology
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