Journal article
Steroids and genes related to steroid biosynthesis in the female giant freshwater prawn, Macrobrachium rosenbergii
Steroids, Vol.107, pp.149-160
2016
Abstract
The giant freshwater prawn, Macrobrachium rosenbergii, is important to many Asian countries due to its high economic value as an aquaculture product. With demand increasing, there is requirement for a better understanding of the biosynthetic components that regulate its growth and reproduction, including steroids, in order to help increase production. Vertebrate-type steroids and their receptors were identified in crustaceans and implicated in reproduction. In this study, we presented the sex steroids estradiol and progesterone by LC-MS/MS in female M. rosenbergii, and reveal steroidogenic-related genes by in silico analysis of de novo assembled transcriptomes. Comparative analysis with other species was performed to confirm their putative role, as well as tissue-specific and quantitative gene expression. We reveal 29 transcripts that encode for steroidogenic-related proteins, including steroidogenic enzymes, a nuclear steroid hormone receptor, and a steroidogenic factor. Moreover, we identified for the first time the presence of steroidogenic factor 1, StAR-related lipid transfer protein, estradiol receptor- and progesterone-like protein in M. rosenbergii. Those targeted for gene expression analysis (3 beta-hydroxysteroid dehydrogenase, 17 beta-hydroxysteroid dehydrogenase, estrogen sulfotransferase and progesterone receptor-like) showed widespread expression within many tissues, and at relatively high levels in the central nervous system during ovarian maturation. In summary, we provide further evidence for the existence of steroidogenic pathways in crustaceans, which may be useful for advancing prawn aquaculture.
Details
- Title
- Steroids and genes related to steroid biosynthesis in the female giant freshwater prawn, Macrobrachium rosenbergii
- Authors
- Tipsuda Thongbuakaew (Author) - Mahidol University, ThailandTanapan Siangcham (Author) - Mahidol University, ThailandSaowaros Suwansa-ard (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringAbigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringScott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringPrasert Sobhon (Author) - Mahidol University, ThailandPrapee Sretarugsa (Author) - Mahidol University, Thailand
- Publication details
- Steroids, Vol.107, pp.149-160
- Publisher
- Elsevier Inc.
- Date published
- 2016
- DOI
- 10.1016/j.steroids.2016.01.006
- ISSN
- 0039-128X
- Copyright note
- Copyright © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449308002621
- Output Type
- Journal article
Metrics
153 File views/ downloads
1032 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Endocrinology & Metabolism
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites