Staphylococcus aureus from patients with chronic rhinosinusitis show minimal genetic association between polyp and non-polyp phenotypes

Jake Jervis Bardy; Derek S Sarovich; Erin P Price; Eike Steinig; Steven Tong; Amanda Drilling; Judy Ou; Sarah Vreugde; Peter-John Wormald; Alkis J Psaltis

doi:10.1186/s12901-018-0064-1

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Staphylococcus aureus from patients with chronic rhinosinusitis show minimal genetic association between polyp and non-polyp phenotypes

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Staphylococcus aureus from patients with chronic rhinosinusitis show minimal genetic association between polyp and non-polyp phenotypes

Jake Jervis Bardy, Derek S Sarovich, Erin P Price, Eike Steinig, Steven Tong, Amanda Drilling, Judy Ou, Sarah Vreugde, Peter-John Wormald and Alkis J Psaltis

BMC Ear, Nose and Throat Disorders, Vol.18, 16

2018

DOI: https://doi.org/10.1186/s12901-018-0064-1

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Abstract

chronic rhinosinusitis

genome-wide association study

microbial genomics

staphylococcus aureus

whole genome sequencing

Background: Staphylococcus aureus has a high prevalence in chronic rhinosinusitis (CRS) patients and is suggested to play a more etiopathogenic role in CRS patients with nasal polyps (CRSwNP), a severe form of the CRS spectrum with poorer surgical outcomes. We performed a microbial genome-wide association study (mGWAS) to investigate whether S. aureus isolates from CRS patients have particular genetic markers associated with CRS with nasal polyps (CRSwNP) or CRS without nasal polyps (CRSsNP). Methods: Whole genome sequencing was performed on S. aureus isolates collected from 28 CRSsNP and 30 CRSwNP patients. A mGWAS approach was employed using large-scale comparative genomics to identify genetic variation within our dataset. Results: Considerable genetic variation was observed, with > 90,000 single nucleotide polymorphisms (SNPs) sites identified. There was little correlation with CRS subtype based on SNPs and Insertion/Delection (Indels). One indel was found to significantly correlate with CRSwNP and occurred in the promoter region of a bacitracin transport system ATP-binding protein. Additionally, two variants of the highly variable superantigen-like (SSL) proteins were found to significantly correlate with each CRS phenotype. No significant association with other virulence or antibiotic resistance genes were observed, consistent with previous studies. Conclusion: To our knowledge this study is the first to use mGWAS to investigate the contribution of microbial genetic variation to CRS presentations. Utilising the most comprehensive genome-wide analysis methods available, our results suggest that CRS phenotype may be influenced by genetic factors other than specific virulence mechanisms within the S. aureus genome.

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Title: Staphylococcus aureus from patients with chronic rhinosinusitis show minimal genetic association between polyp and non-polyp phenotypes
Authors: Jake Jervis Bardy (Author) - University of Adelaide
Derek S Sarovich (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Erin P Price (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Eike Steinig (Author) - James Cook University
Steven Tong (Author) - Charles Darwin University
Amanda Drilling (Author) - University of Adelaide
Judy Ou (Author) - University of Adelaide
Sarah Vreugde (Author) - University of Adelaide
Peter-John Wormald (Author) - University of Adelaide
Alkis J Psaltis (Author) - University of Adelaide
Publication details: BMC Ear, Nose and Throat Disorders, Vol.18, 16; 8
Publisher: BioMed Central Ltd.
Date published: 2018
DOI: 10.1186/s12901-018-0064-1
ISSN: 1472-6815
Copyright note: Copyright © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99450642002621
Output Type: Journal article

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Web Of Science research areas: Otorhinolaryngology

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