Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei

Michael J Stewart; Praphaporn Stewart; N Soonklang; V Linthong; P J Hanna; W Duan; P Sobhon

doi:10.1016/j.tice.2010.03.002

Back

Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei

Journal article

Peer reviewed

Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei

Michael J Stewart, Praphaporn Stewart, N Soonklang, V Linthong, P J Hanna, W Duan and P Sobhon

Tissue and Cell, Vol.42(3), pp.137-150

2010

DOI: https://doi.org/10.1016/j.tice.2010.03.002

Files and links (1)

url

https://doi.org/10.1016/j.tice.2010.03.002View

Published Version

Abstract

Portunus pelagicus

spermatogenesis

spermatozoa

ultrastructure

basic nuclear proteins

Spermatogenesis in the blue swimming crab, Portunus pelagicus, is described by light and electron microscopy. The testis is composed of anterior (AT) and posterior (PT) lobes, that are partitioned into lobules by connective tissue trabecula, and further divided into zones (germinal, transformation and evacuation), each with various stages of cellular differentiation. The vas deferens is classified into three distinct regions: anterior (AVD), median (MVD), and posterior (PVD), on the presence of spermatophores and two secretions, termed substance I and II. Based on the degree and patterns of heterochromatin, spermatogenesis is classified into 13 stages: two spermatogonia (SgA and SgB), six primary spermatocytes (leptotene, zygotene, pachytene, diplotene, diakinesis, and metaphase), a secondary spermatocyte (SSc), three spermatids (St 1-3), and a mature spermatozoon. Spermatid stages are differentiated by chromatin decondensation and the formation of an acrosomal complex, which is unique to brachyurans. Mature spermatozoa are aflagellated, and have a nuclear projection and a spherical acrosome. AUT-PAGE and Western blots show that, during chromatin decondensation, there is a reduction of most histones, with only small amounts of H2B and H3 remaining in mature spermatozoa.

Details

Title: Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei
Authors: Michael J Stewart (Author) - Deakin University
Praphaporn Stewart (Author) - Thammasat University, Thailand
N Soonklang (Author) - Thammasat University, Thailand
V Linthong (Author) - Mahidol University, Thailand
P J Hanna (Author) - Mahidol University, Thailand
W Duan (Author) - Deakin University
P Sobhon (Author) - Mahidol University, Thailand
Publication details: Tissue and Cell, Vol.42(3), pp.137-150
Publisher: Churchill Livingstone
Date published: 2010
DOI: 10.1016/j.tice.2010.03.002
ISSN: 0040-8166
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450258402621
Output Type: Journal article

Metrics

6 File views/ downloads

652 Record Views

55 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Anatomy & Morphology; Cell Biology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites