Journal article
Soluble mediators in packed red blood cells augment lipopolysaccharide‐induced monocyte interleukin‐1β production
Vox Sanguinis, Vol.115(7), pp.562-569
2020
Abstract
Background and objectives: Soluble mediators in packed red-blood-cell (PRBC) units have been hypothesized as a mechanism associated with transfusion-related immune modulation. Soluble mediators including damage-associated molecular patterns (DAMPs) are known to activate inflammasomes. Inflammasome complexes maturate caspase-1 and interleukin (IL)-1β. We assessed whether PRBC supernatants (SN) modulated IL-1β driven inflammation and whether macrophage migration inhibitory factor (MIF) was a contributing factor. Materials and methods: Isolated monocytes were incubated with PRBC-SN in an in vitro transfusion model. Lipopolysaccharide (LPS) was added in parallel to model a bacterial infection. Separately, recombinant MIF was used in the model to assess its role in ILL‐1β driven inflammation. IL-1β and caspase-1 were quantified in the PRBC-SN and culture SN from the in vitro model. Results: PRBC-SN alone did not induce IL-1β production from monocytes. However, PRBC-SN alone increased caspase-1 production. LPS alone induced both IL-1β and caspase-1 production. PRBC-SN augmented LPS-driven IL-1β and caspase-1 production. Recombinant MIF did not modulate IL-1β production in our model. Conclusions: Soluble mediators in PRBC modulate monocyte IL-1β inflammation, which may be a contributing factor to adverse effects of transfusion associated with poor patient outcomes. While MIF was present in PRBC-SN, we found no evidence that MIF was responsible for IL-1β associated immune modulation.
Details
- Title
- Soluble mediators in packed red blood cells augment lipopolysaccharide‐induced monocyte interleukin‐1β production
- Authors
- Fenny Chong (Corresponding Author) - Australian Red Cross LifebloodKelly M Rooks (Author) - Australian Red Cross LifebloodRobert L Flower (Author) - Australian Red Cross LifebloodMelinda M Dean (Author) - University of the Sunshine Coast - School of Health and Sport Sciences
- Publication details
- Vox Sanguinis, Vol.115(7), pp.562-569
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 2020
- DOI
- 10.1111/vox.12915
- ISSN
- 0042-9007; 1423-0410
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99450944402621
- Output Type
- Journal article
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- Hematology
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