Soil urease: Activity, stability and kinetic properties

N M Pettit; A R J Smith; R B Freedman; Richard G Burns

doi:10.1016/0038-0717(76)90089-4

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Soil urease: Activity, stability and kinetic properties

Journal article

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Soil urease: Activity, stability and kinetic properties

N M Pettit, A R J Smith, R B Freedman and Richard G Burns

Soil Biology and Biochemistry, Vol.8(6), pp.479-484

1976

DOI: https://doi.org/10.1016/0038-0717(76)90089-4

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Abstract

soil urease

The activity, stability and kinetic characteristics of soil urease are examined and compared with those of a crude enzyme-organic matter extract from soil and of jack bean urease. Methods of organic matter extraction from soil are discussed and the resistance of all three forms of urease to irradiation, proteolysis, temperature, storage and lyophilization is assessed. In addition, measurements are made of Michaelis constants and pH optima. The data confirm previous observations in that at least a proportion of soil urease is remarkably stable in conditions which rapidly inactivate jack bean urease. Stability is maintained with the enzymically-active organic extract.

Details

Title: Soil urease: Activity, stability and kinetic properties
Authors: N M Pettit (Author) - University of Kent, United Kingdom
A R J Smith (Author) - University of Kent, United Kingdom
R B Freedman (Author) - University of Kent, United Kingdom
Richard G Burns (Author) - University of Kent, United Kingdom
Publication details: Soil Biology and Biochemistry, Vol.8(6), pp.479-484
Publisher: Pergamon
Date published: 1976
DOI: 10.1016/0038-0717(76)90089-4
ISSN: 0038-0717
Organisation Unit: University of the Sunshine Coast, Queensland; Office of the Deputy Vice-Chancellor (Research and Innovation)
Language: English
Record Identifier: 99449489102621
Output Type: Journal article

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