Skeletal muscle microvascular perfusion responses to cuff occlusion and submaximal exercise assessed by contrast‐enhanced ultrasound: The effect of age

Annelise L Meneses; Michael Nam; Tom Bailey; Chris Anstey; Jonathan Golledge; Michelle A Keske; Kim Greaves; Christopher Askew

doi:10.14814/phy2.14580

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Skeletal muscle microvascular perfusion responses to cuff occlusion and submaximal exercise assessed by contrast‐enhanced ultrasound: The effect of age

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Skeletal muscle microvascular perfusion responses to cuff occlusion and submaximal exercise assessed by contrast‐enhanced ultrasound: The effect of age

Annelise L Meneses, Michael Nam, Tom Bailey, Chris Anstey, Jonathan Golledge, Michelle A Keske, Kim Greaves and Christopher Askew

Physiological Reports, Vol.8(19), pp.1-13

2020

DOI: https://doi.org/10.14814/phy2.14580

PMCID: PMC7547535

PMID: 33038050

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Abstract

aging

blood flow

exercise

muscle perfusion

reactive hyperemia

vascular conductance

Impairments in skeletal muscle microvascular function are frequently reported in patients with various cardiometabolic conditions for which older age is a risk factor. Whether aging per se predisposes the skeletal muscle to microvascular dysfunction is unclear. We used contrast‐enhanced ultrasound (CEU) to compare skeletal muscle microvascular perfusion responses to cuff occlusion and leg exercise between healthy young (n = 12, 26 ± 3 years) and older (n = 12, 68 ± 7 years) adults. Test–retest reliability of CEU perfusion parameters was also assessed. Microvascular perfusion (microvascular volume × flow velocity) of the medial gastrocnemius muscle was measured before and immediately after: (a) 5‐min of thigh‐cuff occlusion, and (b) 5‐min of submaximal intermittent isometric plantar‐flexion exercise (400 N) using CEU. Whole‐leg blood flow was measured using strain‐gauge plethysmography. Repeated measures were obtained with a 15‐min interval, and averaged responses were used for comparisons between age groups. There were no differences in post‐occlusion whole‐leg blood flow and muscle microvascular perfusion between young and older participants (p > .05). Similarly, total whole‐leg blood flow during exercise and post‐exercise peak muscle microvascular perfusion did not differ between groups (p > .05). The overall level of agreement between the test–retest measures of calf muscle perfusion was excellent for measurements taken at rest (intraclass correlation coefficient [ICC] 0.85), and in response to cuff occlusion (ICC 0.89) and exercise (ICC 0.95). Our findings suggest that healthy aging does not affect muscle perfusion responses to cuff‐occlusion and submaximal leg exercise. CEU muscle perfusion parameters measured in response to these provocation tests are highly reproducible in both young and older adults.

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Title: Skeletal muscle microvascular perfusion responses to cuff occlusion and submaximal exercise assessed by contrast‐enhanced ultrasound: The effect of age
Authors: Annelise L Meneses (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - Legacy
Michael Nam (Author) - Sunshine Coast University Hospital (Australia)
Tom Bailey (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - Legacy
Chris Anstey (Author) - Sunshine Coast Hospital and Health Service
Jonathan Golledge (Author) - James Cook University
Michelle A Keske (Author) - Deakin University
Kim Greaves (Author) - University of the Sunshine Coast
Christopher Askew (Corresponding Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - Legacy
Publication details: Physiological Reports, Vol.8(19), pp.1-13
Publisher: John Wiley & Sons Ltd.
Date published: 2020
DOI: 10.14814/phy2.14580
PMID: 33038050; PMC7547535
Copyright note: © 2020 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Organisation Unit: Faculty of Science, Health, Education and Engineering; UniSC Clinical Trials Centre; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Cancer Research Cluster; School of Health - Sports & Exercise Science; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99483708102621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Physiology