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Single-nucleotide polymorphisms that uniquely identify cultivars of avocado Persea americana388.37 kB
Published VersionCC BY-NC-ND V4.0, Open Access
Journal article
Single‐nucleotide polymorphisms that uniquely identify cultivars of avocado (Persea americana)
Applications in Plant Sciences, Vol.9(6), pp.1-8
2021
Abstract
Premise:
Progeny of avocado (Persea americana) are highly variable due to high levels of heterozygosity. Breeding programs need molecular resources to allow the assessment of genetic differences and the selection of genotypes. Polymorphisms that uniquely identify different avocado cultivars provide a valuable tool to accelerate avocado research and development, including, for example, genotype selection.
Methods:
A double‐digest restriction site–associated DNA sequencing (ddRADseq) approach was used to screen 10 avocado cultivars for single‐nucleotide polymorphisms (SNPs). The fragments were size selected with Blue Pippin and PCR using universal Illumina primers, and catalog tags were then created with de novo alignment using Stacks software. Catalog tags were tabulated and filtered to identify alleles unique to each cultivar.
Results:
A total of 104 million sequences were collected, and 52 homozygous SNPs were identified that uniquely distinguished nine avocado cultivars. The cultivars Carmen Hass and Hass have a strong genetic similarity and no homozygous SNPs distinguishing these cultivars could be identified; therefore, both cultivars were grouped together.
Discussion:
The resource described here for cultivars of P. americana presents a new and significant molecular resource that can enable targeted genotype selection, paternity analysis, germplasm genotyping, pollination dynamics investigation, and crop improvement.
Details
- Title
- Single‐nucleotide polymorphisms that uniquely identify cultivars of avocado (Persea americana)
- Authors
- Wiebke Kämper (Corresponding Author) - Griffith UniversityStephen J Trueman (Author) - Griffith UniversityJade Cooke (Author) - AGRF LtdNaga Kasinadhuni (Author) - AGRF LtdAaron J Brunton (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacySteven M Ogbourne (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
- Publication details
- Applications in Plant Sciences, Vol.9(6), pp.1-8; 8
- Publisher
- John Wiley & Sons Ltd
- Date published
- 2021
- DOI
- 10.1002/aps3.11440
- ISSN
- 2168-0450
- Copyright note
- © 2021 The Authors. Applications in Plant Sciences published by Wiley Periodicals LLC on behalf of Botanical Society of America. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; Forest Industries Research Centre; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99571607102621
- Output Type
- Journal article
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- Domestic collaboration
- Web Of Science research areas
- Plant Sciences
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