Single-molecule sequencing reveals the molecular basis of multidrug-resistance in ST772 methicillin-resistant Staphylococcus aureus

E J Steinig; P Andersson; Simon R Harris; Derek S Sarovich; A Manoharan; P Coupland; M T G Holden; J Parkhill; S D Bentley; D A Robinson; S Y C Tong

doi:10.1186/s12864-015-1599-9

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Single-molecule sequencing reveals the molecular basis of multidrug-resistance in ST772 methicillin-resistant Staphylococcus aureus

Journal article

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Peer reviewed

Single-molecule sequencing reveals the molecular basis of multidrug-resistance in ST772 methicillin-resistant Staphylococcus aureus

E J Steinig, P Andersson, Simon R Harris, Derek S Sarovich, A Manoharan, P Coupland, M T G Holden, J Parkhill, S D Bentley, D A Robinson, …

BMC Genomics, Vol.16(1), 388

2015

DOI: https://doi.org/10.1186/s12864-015-1599-9

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Abstract

staphylococcus aureus

MRSA

ST772

antibiotic resistance

mobile genetic elements

complete genome

DAR4145

India

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital-associated infection, but there is growing awareness of the emergence of multidrug-resistant lineages in community settings around the world. One such lineage is ST772-MRSA-V, which has disseminated globally and is increasingly prevalent in India. Here, we present the complete genome sequence of DAR4145, a strain of the ST772-MRSA-V lineage from India, and investigate its genomic characteristics in regards to antibiotic resistance and virulence factors. Results: Sequencing using single-molecule real-time technology resulted in the assembly of a single continuous chromosomal sequence, which was error-corrected, annotated and compared to nine draft genome assemblies of ST772-MRSA-V from Australia, Malaysia and India. We discovered numerous and redundant resistance genes associated with mobile genetic elements (MGEs) and known core genome mutations that explain the highly antibiotic resistant phenotype of DAR4145. Staphylococcal toxins and superantigens, including the leukotoxin Panton-Valentinin Leukocidin, were predominantly associated with genomic islands and the phage Ï• -IND772PVL. Some of these mobile resistance and virulence factors were variably present in other strains of the ST772-MRSA-V lineage. Conclusions: The genomic characteristics presented here emphasize the contribution of MGEs to the emergence of multidrug-resistant and highly virulent strains of community-associated MRSA. Antibiotic resistance was further augmented by chromosomal mutations and redundancy of resistance genes. The complete genome of DAR4145 provides a valuable resource for future investigations into the global dissemination and phylogeography of ST772-MRSA-V. © Steinig et al.; licensee BioMed Central.

Details

Title: Single-molecule sequencing reveals the molecular basis of multidrug-resistance in ST772 methicillin-resistant Staphylococcus aureus
Authors: E J Steinig (Author) - Menzies School of Health Research
P Andersson (Author) - Wellcome Trust Sanger Institute, United Kingdom
Simon R Harris (Author) - Menzies School of Health Research
Derek S Sarovich (Author) - Menzies School of Health Research
A Manoharan (Author) - Pushpagiri Institute of Medical Sciences and Research Center, India
P Coupland (Author) - Wellcome Trust Sanger Institute, United Kingdom
M T G Holden (Author) - University of St. Andrews, United Kingdom
J Parkhill (Author) - Wellcome Trust Sanger Institute, United Kingdom
S D Bentley (Author) - Wellcome Trust Sanger Institute, United Kingdom
D A Robinson (Author) - University of Mississippi Medical Center, United States
S Y C Tong (Author) - Menzies School of Health Research
Publication details: BMC Genomics, Vol.16(1), 388; 10
Publisher: BioMed Central Ltd.
Date published: 2015
DOI: 10.1186/s12864-015-1599-9
ISSN: 1471-2164
Copyright note: Copyright © 2015 Steinig et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99450331202621
Output Type: Journal article

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Web Of Science research areas: Biotechnology & Applied Microbiology; Genetics & Heredity

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