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Single-cell sequencing reveals the potential oncogenic expression atlas of human iPSC-derived cardiomyocytes3.68 MB
Published VersionCC BY V4.0, Open Access
Journal article
Single-cell sequencing reveals the potential oncogenic expression atlas of human iPSC-derived cardiomyocytes
Biology Open, Vol.10(2), bio053348
2021
PMID: 33589441
Appears in Cancer Research Cluster Research Collection
Abstract
Human induced pluripotent stem cells (iPSCs) are important source for regenerative medicine. However, the links between pluripotency and oncogenic transformation raise safety issues. To understand the characteristics of iPSC-derived cells at single-cell resolution, we directly reprogrammed two human iPSC lines into cardiomyocytes and collected cells from four time points during cardiac differentiation for single-cell sequencing. We captured 32,365 cells and identified five molecularly distinct clusters that aligned well with our reconstructed differentiation trajectory. We discovered a set of dynamic expression events related to the upregulation of oncogenes and the decreasing expression of tumor suppressor genes during cardiac differentiation, which were similar to the gain-of-function and loss-of-function patterns during oncogenesis. In practice, we characterized the dynamic expression of the
Details
- Title
- Single-cell sequencing reveals the potential oncogenic expression atlas of human iPSC-derived cardiomyocytes
- Authors
- Minglin Ou (Author) - Jinan UniversityMin Zhao (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyChunhong Li (Author) - Francis Crick InstituteDonge Tang (Author) - Jinan UniversityYong Xu (Author) - Chinese Academy of SciencesWeier Dai (Author) - The University of Texas at AustinWeiguo Sui (Author) - Guangxi Key laboratory of Metabolic Diseases Research, Central Laboratory of Guilin No. 181 Hospital, Guilin 541002, ChinaYue Zhang (Author) - Guangxi Key laboratory of Metabolic Diseases Research, Central Laboratory of Guilin No. 181 Hospital, Guilin 541002, ChinaZhen Xiang (Author) - Guangxi Key laboratory of Metabolic Diseases Research, Central Laboratory of Guilin No. 181 Hospital, Guilin 541002, ChinaChune Mo (Author) - Guangxi Key laboratory of Metabolic Diseases Research, Central Laboratory of Guilin No. 181 Hospital, Guilin 541002, ChinaHua Lin (Author) - Guangxi Key laboratory of Metabolic Diseases Research, Central Laboratory of Guilin No. 181 Hospital, Guilin 541002, ChinaYong Dai (Author) - Guangxi Key laboratory of Metabolic Diseases Research, Central Laboratory of Guilin No. 181 Hospital, Guilin 541002, China
- Publication details
- Biology Open, Vol.10(2), bio053348
- Publisher
- Company of Biologists Ltd.
- Date published
- 2021
- DOI
- 10.1242/bio.053348
- ISSN
- 2046-6390
- PMID
- 33589441
- Copyright note
- © 2021. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; Cancer Research Cluster; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99511307102621
- Output Type
- Journal article
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