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Silencing of transcription factor encoding gene StTCP23 by small RNAs derived from the virulence modulating region of potato spindle tuber viroid3.37 MB
Published VersionCC BY V4.0, Open Access
Journal article
Silencing of transcription factor encoding gene StTCP23 by small RNAs derived from the virulence modulating region of potato spindle tuber viroid is associated with symptom development in potato
PLoS Pathogens, Vol.15(12), pp.1-29
2019
PMCID: PMC6907872
PMID: 31790500
Abstract
Viroids are small, non-protein-coding RNAs which can induce disease symptoms in a variety of plant species. Potato (Solanum tuberosum L.) is the natural host of Potato spindle tuber viroid (PSTVd) where infection results in stunting, distortion of leaves and tubers and yield loss. Replication of PSTVd is accompanied by the accumulation of viroid-derived small RNAs (sRNAs) proposed to play a central role in disease symptom development. Here we report that PSTVd sRNAs direct RNA silencing in potato against StTCP23, a member of the TCP (teosinte branched1/Cycloidea/Proliferating cell factor) transcription factor family genes that play an important role in plant growth and development as well as hormonal regulation, especially in responses to gibberellic acid (GA). The StTCP23 transcript has 21-nucleotide sequence complementarity in its 3' untranslated region with the virulence-modulating region (VMR) of PSTVd strain RG1, and was downregulated in PSTVd-infected potato plants. Analysis using 3' RNA ligase-mediated rapid amplification of cDNA ends (3' RLM RACE) confirmed cleavage of StTCP23 transcript at the expected sites within the complementarity with VMR-derived sRNAs. Expression of these VMR sRNA sequences as artificial miRNAs (amiRNAs) in transgenic potato plants resulted in phenotypes reminiscent of PSTVd-RG1-infected plants. Furthermore, the severity of the phenotypes displayed was correlated with the level of amiRNA accumulation and the degree of amiRNA-directed down-regulation of StTCP23. In addition, virus-induced gene silencing (VIGS) of StTCP23 in potato also resulted in PSTVd-like phenotypes. Consistent with the function of TCP family genes, amiRNA lines in which StTCP23 expression was silenced showed a decrease in GA levels as well as alterations to the expression of GA biosynthesis and signaling genes previously implicated in tuber development. Application of GA to the amiRNA plants minimized the PSTVd-like phenotypes. Taken together, our results indicate that sRNAs derived from the VMR of PSTVd-RG1 direct silencing of StTCP23 expression, thereby disrupting the signaling pathways regulating GA metabolism and leading to plant stunting and formation of small and spindle-shaped tubers.
Details
- Title
- Silencing of transcription factor encoding gene StTCP23 by small RNAs derived from the virulence modulating region of potato spindle tuber viroid is associated with symptom development in potato
- Authors
- Sarina Bao (Author) - Inner Mongolia UniversityRobert A Owens (Author) - United States Department of AgricultureQinghua Sun (Author) - Inner Mongolia UniversityHonglin Song (Author) - Inner Mongolia UniversityYanan Liu (Author) - Inner Mongolia UniversityAndrew Eamens (Author) - University of Newcastle AustraliaHao Feng (Author) - Inner Mongolia UniversityHongzhi Tian (Author) - Inner Mongolia UniversityMing-Bo Wang (Author) - CSIRO Agriculture and Food (Australia)Ruofang Zhang (Author) - Inner Mongolia University
- Publication details
- PLoS Pathogens, Vol.15(12), pp.1-29
- Publisher
- Public Library of Science
- Date published
- 2019
- DOI
- 10.1371/journal.ppat.1008110
- ISSN
- 1553-7374; 1553-7366
- PMID
- 31790500; PMC6907872
- Copyright note
- Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99656592502621
- Output Type
- Journal article
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- Web Of Science research areas
- Microbiology
- Parasitology
- Virology
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