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Shelf-life of a biocontrol Pseudomonas putida applied to sugar beet seeds using commercial coatings
Journal article   Peer reviewed

Shelf-life of a biocontrol Pseudomonas putida applied to sugar beet seeds using commercial coatings

D A Shah-Smith and Richard G Burns
Biocontrol Science and Technology, Vol.7(1), pp.65-74
1997
DOI: https://doi.org/10.1080/09583159731054
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https://doi.org/10.1080/09583159731054View
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Abstract

biological control damping off film-coats Pseudomonas putida Pythium ultimum seed pellets shelf-life
Pseudomonas putida 40RNF is a putative biological control agent (BCA) of Pythium damping-off of sugar beet. The survival of 40RNF during commercial seed treatment and its subsequent shelf-life (i.e. long-term viability and biocontrol activity) were assessed. Two methods were used to apply 40RNF to sugar beet seeds: incorporation into film-coats sprayed on to pre-pelleted seeds and incorporation into the pellet material prior to pelleting. Only 7.1% of applied 40RNF survived film-coating, but an initial concentration of 7 x lOs ensured that 83.3% of a pre-deter-mined target rate of 6 x 107/pellet was achieved. After 52 weeks of storage at 4°C, the numbers of 40RNF had declined by one to two orders of magnitude, with a decrease of approximately 50% in disease control. After 52 weeks at 18-20°C, 40RNF was below detectable limits (< 100/pellet), yet the biocontrol activity of the seed treatments was not reduced. The survival of 40RNF during incorporation into the pellet material was poor (< 0.2% of those applied, i.e. 5 x 105 pellet). However, bacterial viability and biocontrol efficacy were maintained at 100% of the control value for 24 weeks when stored at 18-20°C. The results indicate that commercial seed treatments and the storage of pellets at ambient temperatures has potential for the introduction of bacterial BCAs into the spermosphere.

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