Sex change strategy and the aromatase genes

Luke Gardner; Trevor A Anderson; A R Place; B Dixon; Abigail Elizur

doi:10.1016/j.jsbmb.2004.12.045

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Sex change strategy and the aromatase genes

Journal article

Peer reviewed

Sex change strategy and the aromatase genes

Luke Gardner, Trevor A Anderson, A R Place, B Dixon and Abigail Elizur

Journal of Steroid Biochemistry and Molecular Biology, Vol.94(5), pp.395-404

2005

DOI: https://doi.org/10.1016/j.jsbmb.2004.12.045

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Abstract

Fisheries Sciences

cytochrome P450 aromatase

CYP19

promoters

sex change

Lates calcarifer

Cromileptes altivelis

Gobiodon histrio

Sequential hermaphroditism is a common reproductive strategy in many teleosts. Steroid production is known to mediate both the natural and induced sex change, yet beyond this the physiology directing this process has received little attention. Cytochrome P450 aromatase is a key enzyme in the hormonal pathway catalysing the conversion of sex steroids, androgens to oestrogens, and thus is highly relevant to the process of sex change. This study reports the isolation of cDNA sequences for aromatase isoforms CYP19A1 and CYP19A2 from teleost species representing three forms of sexual hermaphroditism: Lates calcarifer (protandry), Cromileptes altivelis (protogyny), and Gobiodon histrio (bi-directional). Deduced amino acid analysis of these isoforms with other reported isoforms from gonochoristic (single sex) teleosts revealed 56-95% identity within the same isoform while only 48-65% identity between isoforms irrespective of species and sexual strategy. Phylogenetic analysis supported this result separating sequences into isoform exclusive clades in spite of species apparent evolutionary distance. Furthermore, this study isolates 5â€² flanking regions of all above genes and describes putative cis-acting elements therein. Elements identified include steroidogenic factor 1 binding site (SF-1), oestrogen response element (ERE), progesterone response element (PRE), androgen response element (ARE), glucocorticoid response elements (GRE), peroxisome proliferator-activated receptor α/retinoid X receptor α heterodimer responsive element (PPARα/RXRα), nuclear factor kappaβ (NF-kappaβ), SOX 5, SOX 9, and Wilms tumor suppressor (WTI). A hypothetical in vivo model was constructed for both isoforms highlighting potential roles of these putative cis-acting elements with reference to normal function and sexual hermaphroditism.

Details

Title: Sex change strategy and the aromatase genes
Authors: Luke Gardner (Author) - Queensland Department of Primary Industries & Fisheries
Trevor A Anderson (Author) - James Cook University
A R Place (Author) - University of Maryland, United States
B Dixon (Author) - Queensland Department of Primary Industries & Fisheries
Abigail Elizur (Author) - Queensland Department of Primary Industries & Fisheries
Publication details: Journal of Steroid Biochemistry and Molecular Biology, Vol.94(5), pp.395-404
Publisher: Pergamon
Date published: 2005
DOI: 10.1016/j.jsbmb.2004.12.045
ISSN: 0960-0760
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449540602621
Output Type: Journal article

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Web Of Science research areas: Biochemistry & Molecular Biology; Endocrinology & Metabolism

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