Journal article
Serological assessment of chlamydial infection in the koala by a slide EIA technique
Australian Veterinary Journal, Vol.68(12), pp.393-396
1991
Abstract
A rapid and simplified slide enzyme immunosorbent assay (EIA) was developed for the diagnosis of chlamydial infection in the koala. HeLa 229 cells infected with koala strain Chlamydia psittaci were fixed on the surface of multiwell slides and used as the antigen. The assay consisted of first reacting koala antiserum with the fixed C psittaci antigen, followed by reaction with biotinylated rabbit anti-koala IgG, ABC reagent and substrate. The chlamydial EIA antibody titres obtained were compared with those of a complement fixation (CF) test using koala strain C psittaci as antigen. Of 35 koala sera tested, 16 CF positive sera (greater than or equal to 1:8) also had a positive titre (greater than or equal to 1:200) in the slide EIA test (sensitivity 93.8%, 15/16). Nineteen CF negative sera were also negative in the slide EIA (specificity 100%, 19/19). Sixty-eight samples of koala blood were collected by ear-prick using a sampling paper method and were assayed by both tests. Sensitivity of the slide EIA was 100% (15/15) and specificity of the test was 96.2% (51/53). To simplify the slide EIA for use as a practical screening test, a 3-point serum dilution series (1:100, 1:200, 1:400) was used. This 3-point slide EIA was compared with the CF test using sheep strain chlamydial antigen. Thirty-nine sera were assayed by both tests. The sensitivity of the 3-point method was 85.7% (6/7) and the specificity was 71.9% (23/32) as compared with the sheep antigen CF test.(ABSTRACT TRUNCATED AT 250 WORDS)
Details
- Title
- Serological assessment of chlamydial infection in the koala by a slide EIA technique
- Authors
- H Ueno (Author) - Rakuno Gakuen University, JapanS Mizuno (Author) - Hokkaido University, JapanI Takashima (Author) - Rakuno Gakuen University, JapanR Osawa (Author) - Lone Pine Koala SanctuaryW Blanshard (Author) - Lone Pine Koala SanctuaryPeter Timms (Author) - Queensland University of TechnologyN White (Author) - Queensland University of TechnologyN Hashimoto (Author) - Hokkaido University, Japan
- Publication details
- Australian Veterinary Journal, Vol.68(12), pp.393-396
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 1991
- DOI
- 10.1111/j.1751-0813.1991.tb03107.x
- ISSN
- 0005-0423
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449897402621
- Output Type
- Journal article
Metrics
555 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Veterinary Sciences
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites