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Published VersionCC BY-NC-ND V4.0, Open Access
Journal article
Sequenced Somatic Cell Reprogramming and Differentiation Inside Nested Hydrogel Droplets
Advanced Biosystems, Vol.4, pp.1-21
2020
Abstract
The efficient genesis of pluripotent cells or therapeutic cells for regenerative medicine involves several external manipulations and conditioning protocols, which drives down clinical applicability. Automated programming of the genesis by microscale physical forces and chronological biochemistry can increase clinical success. The design and fabrication of nested polysaccharide droplets (millimeter-sized) with cell sustaining properties of natural tissues and intrinsic properties for time and space evolution of cell transformation signals between somatic cells, pluripotent cells and differentiated therapeutic cells in a swift and efficient manner without the need for laborious external manipulation are reported. Cells transform between phenotypic states by having single and double nested droplets constituted with extracellular matrix proteins and reprogramming, and differentiation factors infused chronologically across the droplet space. The cell transformation into germ layer cells and bone cells is successfully tested in vitro and in vivo and promotes the formation of new bone tissues. Thus, nested droplets with BMP-2 loaded guests synthesize mineralized bone tissue plates along the length of a cranial non-union bone defect at 4 weeks. The advantages of sequenced somatic cell reprogramming and differentiation inside an individual hydrogel module without external manipulation, promoted by formulating tissue mimetic physical, mechanical, and chemical microenvironments are shown.
Details
- Title
- Sequenced Somatic Cell Reprogramming and Differentiation Inside Nested Hydrogel Droplets
- Authors
- David W Green (Corresponding Author) - University of BirminghamJolanta Watson (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyGregory Watson (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyArtemis Stamboulis (Author) - University of Birmingham
- Publication details
- Advanced Biosystems, Vol.4, pp.1-21
- Publisher
- Wiley-VCH Verlag GmbH & Co. KGaA
- Date published
- 2020
- DOI
- 10.1002/adbi.202000071
- ISSN
- 2366-7478; 2366-7478
- Copyright note
- Copyright © 2020 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99466008302621
- Output Type
- Journal article
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- Materials Science, Biomaterials
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