Journal article
Sequence and gene content of a large fragment of a lizard sex chromosome and evaluation of candidate sex differentiating gene R-spondin 1
BMC Genomics, Vol.14, 899
2013
Abstract
Background: Scant genomic information from non-avian reptile sex chromosomes is available, and for only a few lizards, several snakes and one turtle species, and it represents only a small fraction of the total sex chromosome sequences in these species.Results: We report a 352 kb of contiguous sequence from the sex chromosome of a squamate reptile, Pogona vitticeps, with a ZZ/ZW sex microchromosome system. This contig contains five protein coding genes (oprd1, rcc1, znf91, znf131, znf180), and major families of repetitive sequences with a high number of copies of LTR and non-LTR retrotransposons, including the CR1 and Bov-B LINEs. The two genes, oprd1 and rcc1 are part of a homologous syntenic block, which is conserved among amniotes. While oprd1 and rcc1 have no known function in sex determination or differentiation in amniotes, this homologous syntenic block in mammals and chicken also contains R-spondin 1 (rspo1), the ovarian differentiating gene in mammals. In order to explore the probability that rspo1 is sex determining in dragon lizards, genomic BAC and cDNA clones were mapped using fluorescence in situ hybridisation. Their location on an autosomal microchromosome pair, not on the ZW sex microchromosomes, eliminates rspo1 as a candidate sex determining gene in P. vitticeps.Conclusion: Our study has characterized the largest contiguous stretch of physically mapped sex chromosome sequence (352 kb) from a ZZ/ZW lizard species. Although this region represents only a small fraction of the sex chromosomes of P. vitticeps, it has revealed several features typically associated with sex chromosomes including the accumulation of large blocks of repetitive sequences. © 2013 Ezaz et al.; licensee BioMed Central Ltd.
Details
- Title
- Sequence and gene content of a large fragment of a lizard sex chromosome and evaluation of candidate sex differentiating gene R-spondin 1
- Authors
- T Ezaz (Author) - University of CanberraB Azad (Author) - University of CanberraDenis O'Meally (Author) - University of CanberraM J Young (Author) - University of CanberraK Matsubara (Author) - University of CanberraM J Edwards (Author) - University of CanberraX Zhang (Author) - University of CanberraC E Holleley (Author) - University of CanberraJ E Deakin (Author) - University of CanberraJ A Marshall Graves (Author) - University of CanberraA Georges (Author) - University of CanberraS V Edwards (Author) - Harvard University, United StatesS D Sarre (Author) - University of Canberra
- Publication details
- BMC Genomics, Vol.14, 899; 13
- Publisher
- BioMed Central Ltd.
- Date published
- 2013
- DOI
- 10.1186/1471-2164-14-899
- ISSN
- 1471-2164
- Copyright note
- Copyright © 2013 Ezaz et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Organisation Unit
- University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99450604002621
- Output Type
- Journal article
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