Journal article
Second-generation sequencing of entire mitochondrial coding-regions (∼15.4 kb) holds promise for study of the phylogeny and taxonomy of human body lice and head lice
Medical and Veterinary Entomology, Vol.28(Supplement 1), pp.40-50
2014
Abstract
The Illumina Hiseq platform was used to sequence the entire mitochondrial coding-regions of 20 body lice, Pediculus humanus Linnaeus, and head lice, P. capitis De Geer (Phthiraptera: Pediculidae), from eight towns and cities in five countries: Ethiopia, France, China, Australia and the U.S.A. These data (∼310 kb) were used to see how much more informative entire mitochondrial coding-region sequences were than partial mitochondrial coding-region sequences, and thus to guide the design of future studies of the phylogeny, origin, evolution and taxonomy of body lice and head lice. Phylogenies were compared from entire coding-region sequences (∼15.4 kb), entire cox1 (∼1.5 kb), partial cox1 (∼700 bp) and partial cytb (∼600 bp) sequences. On the one hand, phylogenies from entire mitochondrial coding-region sequences (∼15.4 kb) were much more informative than phylogenies from entire cox1 sequences (∼1.5 kb) and partial gene sequences (∼600 to ∼700 bp). For example, 19 branches had >95% bootstrap support in our maximum likelihood tree from the entire mitochondrial coding-regions (∼15.4 kb) whereas the tree from 700 bp cox1 had only two branches with bootstrap support >95%. Yet, by contrast, partial cytb (∼600 bp) and partial cox1 (∼486 bp) sequences were sufficient to genotype lice to Clade A, B or C. The sequences of the mitochondrial genomes of the P. humanus, P. capitis and P. schaeffi Fahrenholz studied are in NCBI GenBank under the accession numbers KC660761-800, KC685631-6330, KC241882-97, EU219988-95, HM241895-8 and JX080388-407.
Details
- Title
- Second-generation sequencing of entire mitochondrial coding-regions (∼15.4 kb) holds promise for study of the phylogeny and taxonomy of human body lice and head lice
- Authors
- H Xiong (Author) - University of QueenslandD Campelo (Author) - University of QueenslandR J Pollack (Author) - IdentifyUS LLC, United StatesD Raoult (Author) - Aix-MarseilleUniversité, FranceRenfu Shao (Author) - University of QueenslandM Alem (Author) - University of Gondar, EthiopiaJ Ali (Author) - University of Gondar, EthiopiaK Bilcha (Author) - University of Gondar, EthiopiaS C Barker (Author) - University of Queensland
- Publication details
- Medical and Veterinary Entomology, Vol.28(Supplement 1), pp.40-50
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 2014
- DOI
- 10.1111/mve.12076
- ISSN
- 0269-283X
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99448866402621
- Output Type
- Journal article
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- Entomology
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