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Sea anemone-anemonefish symbiosis: Behavior and mucous protein profiling
Journal article   Open access   Peer reviewed

Sea anemone-anemonefish symbiosis: Behavior and mucous protein profiling

Hai-Thanh T Nguyen, Min Zhao, Tianfang Wang, Binh T Dang, Audrey J Geffen and Scott F Cummins
Journal of Fish Biology, Vol.105(2), pp.603-618
2024
PMID: 38747400
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Journal of Fish Biology - 2024 - Nguyen - Sea anemone anemonefish symbiosis Behavior and mucous protein profiling1.88 MBDownloadView
Published Version Open Access CC BY-NC-ND V4.0

Abstract

proteomics acclimation symbiosis skin camouflage clownfish sea anemone mucus
Fish species of the genus Amphiprion (Perciformes: Pomacentridae) seek protection from predators among the tentacles of sea anemones as their natural habitat, where they live essentially unharmed from stinging by the host's nematocysts. The skin mucus of these anemonefish has been suggested as a protective mechanism that prevents the discharge of the nematocysts upon contact. Whereas some anemonefish species seem to produce their own protective mucous coating, others may acquire mucus (or biomolecules within) from the sea anemone during an acclimation period. In controlled experiments, we show that Amphiprion ocellaris acclimated successfully to their natural host anemone species Stichodactyla gigantea, and also to Stichodactyla haddoni, and in some cases Heteractis crispa, neither of which are natural host species. No symbiosis was observed for three other anemone species tested, Entacmaea quadricolor, Macrodactyla doreensis, and Heteractis malu. We explored the skin mucous protein profile from naive and experienced A. ocellaris during their acclimation to natural and unnatural host anemones. We confidently report the presence of metabolic and structural proteins in the skin mucus of all samples, likely involved in immunological defense, molecular transport, stress response, and signal transduction. For those anemonefish that established symbiosis, there was a clear increase in ribosomal-type proteins. We additionally provide evidence for the presence of anemone proteins only in the skin mucus of individuals that established symbiosis. Our results support previous speculation of the role of skin mucous-associated proteins in anemonefish-anemone symbiosis. Further exploration of these mucosal proteins could reveal the mechanism of anemonefish acclimation to host anemones.

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Fisheries
Marine & Freshwater Biology

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