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Published VersionCC BY V4.0, Open Access
Journal article
Screening and isolation of milk-clotting enzymes from seaweed
Journal of Applied Phycology, Vol.36, pp.887-896
2024
Abstract
Cheese-making involves milk coagulation as a crucial step where calf rennet has been used traditionally as the milk-clotting enzyme in the cheese industry. This study investigated milk-clotting enzymes from seven species of seaweed and evaluated the parameters for their isolation and partial purification including post-harvest processing, extraction and purification methods. The saturation degree of ammonium sulphate and the precipitation stages were evaluated to obtain optimal purification conditions, and three desalting methods, namely dialysis, desalting column and the combination of dialysis and desalting column, were investigated to determine the most suitable method for ammonium sulphate removal. Protein extracts of all seven species of seaweeds exhibited some caseinolytic activity, but the extract of unprocessed (whole) dried samples had higher protein yields and stronger caseinolytic activities. The extract from one species, Gracilaria edulis, demonstrated the ability to clot milk. The evaluation of the purification method for G. edulis extract revealed an optimum single step of 50% saturation and ammonium sulphate precipitation with dialysis as the desalting method.
Details
- Title
- Screening and isolation of milk-clotting enzymes from seaweed
- Authors
- Ariestya Arlene Arbita (Author) - UNSW AustraliaNicholas A Paul (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringJulian Cox (Author) - UNSW AustraliaJian Zhao (Corresponding Author) - UNSW Sydney
- Publication details
- Journal of Applied Phycology, Vol.36, pp.887-896
- Publisher
- Springer Dordrecht
- Date published
- 2024
- DOI
- 10.1007/s10811-023-02975-4
- ISSN
- 1573-5176; 0921-8971
- Copyright note
- This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99723798102621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Marine & Freshwater Biology