Saving the sea cucumbers: Using population genomic tools to inform fishery and conservation management of the Fijian sandfish Holothuria (Metriatyla) scabra

Kelly Thomas Brown; Paul C Southgate; Chinthaka A Hewavitharane; Monal Lal

doi:10.1371/journal. pone.0274245

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Saving the sea cucumbers: Using population genomic tools to inform fishery and conservation management of the Fijian sandfish Holothuria (Metriatyla) scabra

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Saving the sea cucumbers: Using population genomic tools to inform fishery and conservation management of the Fijian sandfish Holothuria (Metriatyla) scabra

Kelly Thomas Brown, Paul C Southgate, Chinthaka A Hewavitharane and Monal Lal

PLoS One, Vol.17(9), pp.1-21

2022

DOI: https://doi.org/10.1371/journal. pone.0274245

PMCID: PMC9462726

PMID: 36084062

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Abstract

The ovate family protein named MaOFP1 was identified in banana (Musa acuminata L.AAA) fruit by a yeast two-hybrid (Y2H) method using the banana MADS-box gene MuMADS1 as bait and a 2 day postharvest (DPH) banana fruit cDNA library as prey. The interaction between MuMADS1 and MaOFP1 was further confirmed by Y2H and Bimolecular Fluorescence Complementation (BiFC) methods, which showed that the MuMADS1 K domain interacted with MaOFP1. Real-time quantitative PCR evaluation of MuMADS1 and MaOFP1 expression patterns in banana showed that they are highly expressed in 0 DPH fruit, but present in low levels in the stem, which suggests that simultaneous but different expression patterns exist for both MuMADS1 and MaOFP1 in different tissues and developing fruits. Meanwhile, MuMADS1 and MaOFP1 expression was highly stimulated and greatly suppressed, respectively, by exogenous ethylene. In contrast, MaOFP1 expression was highly stimulated while MuMADS1 was greatly suppressed by the ethylene competitor 1-methylcyclopropene (1-MCP). These results indicate that MuMADS1 and MaOFP1 are antagonistically regulated by ethylene and might play important roles in postharvest banana fruit ripening.

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Title: Saving the sea cucumbers: Using population genomic tools to inform fishery and conservation management of the Fijian sandfish Holothuria (Metriatyla) scabra
Authors: Kelly Thomas Brown (Author) - University of the South Pacific
Paul C Southgate (Author) - University of the Sunshine Coast, Queensland, Australian Centre for Pacific Islands Research
Chinthaka A Hewavitharane (Author) - University of the South Pacific
Monal Lal (Author) - University of the Sunshine Coast, Queensland, Australian Centre for Pacific Islands Research
Publication details: PLoS One, Vol.17(9), pp.1-21
Publisher: Public Library of Science
Date published: 2022
DOI: 10.1371/journal. pone.0274245
ISSN: 1932-6203
PMID: 36084062; PMC9462726
Copyright note: Copyright: © 2022 Brown et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Organisation Unit: Australian Centre for Pacific Islands Research; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99673798902621
Output Type: Journal article

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