Safety and immunogenicity of a prototype anti-Chlamydia pecorum recombinant protein vaccine in lambs and pregnant ewes

Marion Desclozeaux; Martina Jelocnik; Katrina Whitting; Siamak Saifzadeh; Sankhya Bommana; Andrew Potter; Volker Gerdts; Peter Timms; Adam Polkinghorne

doi:10.1016/j.vaccine.2017.03.091

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Safety and immunogenicity of a prototype anti-Chlamydia pecorum recombinant protein vaccine in lambs and pregnant ewes

Journal article

Peer reviewed

Safety and immunogenicity of a prototype anti-Chlamydia pecorum recombinant protein vaccine in lambs and pregnant ewes

Marion Desclozeaux, Martina Jelocnik, Katrina Whitting, Siamak Saifzadeh, Sankhya Bommana, Andrew Potter, Volker Gerdts, Peter Timms and Adam Polkinghorne

Vaccine, Vol.35(27), pp.3461-3465

2017

DOI: https://doi.org/10.1016/j.vaccine.2017.03.091

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Abstract

chlamydia

vaccine

pecorum

lamb

immunoglobulin

IFNγ

Arthritis and kerato-conjunctivitis caused by Chlamydia pecorum in lambs are difficult to diagnose and treat. We tested the ability of a prototype C. pecorum vaccine (SC-vaccine), comprised of C. pecorum major outer membrane protein (MOMP-G) and polymorphic membrane protein G (PmpG), to trigger a Chlamydia-specific humoral and cell-mediated immune response in lambs and pregnant ewes. Vaccinations with the SC-vaccine (one and two injections) were very well tolerated by all ewes and lambs. Although the overall immune responses of ewes to SC-vaccination was poor, their lambs showed stronger antigen-specific immune response than lambs from control vaccine ewes. SC-vaccination in lambs triggered production of systemic anti-MOMP-G and anti-PmpG IgG antibodies and secretory IgA in the ocular mucosa. Double vaccination caused statistically significant increases in the height and duration of the humoral response. Antigen-specific IFN-γ was produced in the peripheral blood mononuclear cells of vaccinated lambs.

Details

Title: Safety and immunogenicity of a prototype anti-Chlamydia pecorum recombinant protein vaccine in lambs and pregnant ewes
Authors: Marion Desclozeaux (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Martina Jelocnik (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Katrina Whitting (Author) - Queensland University of Technology
Siamak Saifzadeh (Author) - Queensland University of Technology
Sankhya Bommana (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Andrew Potter (Author) - University of Saskatchewan, Canada
Volker Gerdts (Author) - University of Saskatchewan, Canada
Peter Timms (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Adam Polkinghorne (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Vaccine, Vol.35(27), pp.3461-3465
Publisher: Elsevier Ltd.
Date published: 2017
DOI: 10.1016/j.vaccine.2017.03.091
ISSN: 0264-410X
Organisation Unit: School of Education and Tertiary Access; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99451090402621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Immunology; Medicine, Research & Experimental

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