SNP Marker Discovery in Koala TLR Genes

Jian Cui; Greta J Frankham; Rebecca N Johnson; Adam Polkinghorne; Peter Timms; Denis O'Meally; Yuanyuan Cheng; Katherine Belov

doi:10.1371/journal.pone.0121068

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SNP Marker Discovery in Koala TLR Genes

Jian Cui, Greta J Frankham, Rebecca N Johnson, Adam Polkinghorne, Peter Timms, Denis O'Meally, Yuanyuan Cheng and Katherine Belov

PLoS One, Vol.10(3), e0121068

2015

DOI: https://doi.org/10.1371/journal.pone.0121068

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Abstract

koala TLR genes

Toll-like receptors (TLRs) play a crucial role in the early defence against invading pathogens, yet our understanding of TLRs in marsupial immunity is limited. Here, we describe the characterisation of nine TLRs from a koala immune tissue transcriptome and one TLR from a draft sequence of the koala genome and the subsequent development of an assay to study genetic diversity in these genes.We surveyed genetic diversity in 20 koalas from New South Wales, Australia and showed that one gene, TLR10 is monomorphic, while the other nine TLR genes have between two and 12 alleles. 40 SNPs (16 non-synonymous) were identified across the ten TLR genes. These markers provide a springboard to future studies on innate immunity in the koala, a species under threat from two major infectious diseases.

Details

Title: SNP Marker Discovery in Koala TLR Genes
Authors: Jian Cui (Author) - University of Sydney
Greta J Frankham (Author) - Australian Museum Research Institute
Rebecca N Johnson (Author) - Australian Museum Research Institute
Adam Polkinghorne (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Peter Timms (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Denis O'Meally (Author) - University of Sydney
Yuanyuan Cheng (Author) - University of Sydney
Katherine Belov (Author) - University of Sydney
Publication details: PLoS One, Vol.10(3), e0121068; 15
Publisher: Public Library of Science
Date published: 2015
DOI: 10.1371/journal.pone.0121068
ISSN: 1932-6203; 1932-6203
Copyright note: Copyright © 2015 Cui et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449951602621
Output Type: Journal article
Research Statement: false

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Collaboration types: Domestic collaboration
Web Of Science research areas: Immunology

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