Riboflavin and ultraviolet light: impact on dengue virus infectivity

Helen M Faddy; J J Fryk; D Watterson; P R Young; N Modhiran; D A Muller; S D Keil; R P Goodrich; D C Marks

doi:10.1111/vox.12414

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Riboflavin and ultraviolet light: impact on dengue virus infectivity

Journal article

Peer reviewed

Riboflavin and ultraviolet light: impact on dengue virus infectivity

Helen M Faddy, J J Fryk, D Watterson, P R Young, N Modhiran, D A Muller, S D Keil, R P Goodrich and D C Marks

Vox Sanguinis, Vol.111(3), pp.235-241

2016

DOI: https://doi.org/10.1111/vox.12414

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https://doi.org/10.1111/vox.12414View

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Abstract

Clinical Sciences

Medical Physiology

dengue

emerging infectious disease

pathogen inactivation

safety

transfusion

Background: Dengue viruses (DENV 1-4) are emerging across the world, and these viruses pose a risk to transfusion safety. Pathogen inactivation may be an alternative approach for managing the risk of DENV transfusion transmission. This study aimed to investigate the ability of riboflavin and UV light to inactivate DENV 1-4 in platelet concentrates. Materials and Methods: DENV 1-4 were spiked into buffy coat-derived platelet concentrates in additive solution (SSP+) before being treated with riboflavin and UV light. Infectious virus was quantified pre-and posttreatment, and the reduction in viral infectivity was calculated. Results: All four DENV serotypes were modestly reduced after treatment. The greatest amount of reduction in infectivity was observed for DENV-4 (1.81 log reduction) followed by DENV-3 (1.71 log reduction), DENV-2 (1.45 log reduction) and then DENV-1 (1.28 log reduction). Conclusion: Our study demonstrates that DENV 1-4 titres are modestly reduced following treatment with riboflavin and UV light. With the increasing number of transfusion-transmitted cases of DENV around the globe, and the increasing incidence and geographical distribution of DENV, additional approaches for maintaining blood safety may be required in the future.

Details

Title: Riboflavin and ultraviolet light: impact on dengue virus infectivity
Authors: Helen M Faddy (Corresponding Author) - Australian Red Cross Blood Service
J J Fryk (Author) - Australian Red Cross Blood Service
D Watterson (Author) - University of Queensland
P R Young (Author) - University of Queensland
N Modhiran (Author) - University of Queensland
D A Muller (Author) - University of Queensland
S D Keil (Author) - Terumo BCT, United States
R P Goodrich (Author) - Terumo BCT, United States
D C Marks (Author) - Australian Red Cross Blood Service
Publication details: Vox Sanguinis, Vol.111(3), pp.235-241
Publisher: Wiley-Blackwell Publishing Ltd.
DOI: 10.1111/vox.12414
ISSN: 0042-9007; 1423-0410
Organisation Unit: School of Health and Behavioural Sciences - Legacy; School of Health and Sport Sciences - Legacy; University of the Sunshine Coast, Queensland; School of Health - Biomedicine
Language: English
Record Identifier: 99450967602621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Hematology

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