Reversible detection of proteases and their inhibitors by a pulsed chronopotentiometric polyion-sensitive electrode

Y Xu; A Shvarev; S Makarychev-Mikhailov; Eric Bakker

doi:10.1016/j.ab.2007.10.043

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Reversible detection of proteases and their inhibitors by a pulsed chronopotentiometric polyion-sensitive electrode

Journal article

Open access

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Reversible detection of proteases and their inhibitors by a pulsed chronopotentiometric polyion-sensitive electrode

Y Xu, A Shvarev, S Makarychev-Mikhailov and Eric Bakker

Analytical Biochemistry, Vol.374(2), pp.366-370

2008

DOI: https://doi.org/10.1016/j.ab.2007.10.043

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Abstract

protamine

trypsin

membrane electrode

pulsed chronopotentiometry

enzyme detection

Polymer membrane electrodes operated by pulsed chronopotentiometry have recently been introduced to replace traditional ion-selective electrodes for a number of applications. While ion-selective electrodes for the polycation protamine have been reported, for instance, a pulsed chronopotentiometric readout mode (called here pulstrode) provides improved stability and reproducibility while exhibiting sufficient selectivity for the direct detection of protamine in undiluted whole blood samples. Here, such protamine-sensitive pulstrodes are applied for the real-time detection of the activity of the protease trypsin and its soybean inhibitor. This is possible because small fragments produced by the trypsin digestion are not detectable by the protamine-sensing membrane. The real-time response to the proteolytic reaction is shown to exhibit good reproducibility and reversibility, and the initial reaction rate is dependent on the concentration of the protease and its inhibitor.

Details

Title: Reversible detection of proteases and their inhibitors by a pulsed chronopotentiometric polyion-sensitive electrode
Authors: Y Xu (Author) - Purdue University, United States
A Shvarev (Author) - Oregon State University, United States
S Makarychev-Mikhailov (Author) - Purdue University, United States
Eric Bakker (Author) - Curtin University
Publication details: Analytical Biochemistry, Vol.374(2), pp.366-370
Publisher: Elsevier Inc.
Date published: 2008
DOI: 10.1016/j.ab.2007.10.043
ISSN: 0003-2697
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450380202621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical